The last couple batches of RPMI 1640 media I have prepared have not been the correct color after the addition of FBS. When aliquoting small volumes before the addition of FBS, I have the expected pink color however once the FBS is added (10%, 50 mL into 500 total mL) the color is a faint red. This color change most likely signifies some kind of pH change, but I'm wondering if the FBS is causing it and why now but not previously?
The media has no contamination, I've left it at room temperature and in the incubator overnight and found no growth.
Is this a new batch of FBS too ? I would expect a lot of difference from one batch of FBS to another, and that certainly includes pH. This faint red RPMI color after addition of FBS was actually what I obtained most of the time. Pink color was usually on old RPMI stored quite a long time.
Could be the temp of your fbs was cold.. temp=pH.. if warmed up does it change ?
Daer Shawn,
I believe that you must control the ph in first term and secondly to test a cell culture with these characteristics that you mention to see the cellular development.
Hi Shawn Mcginley
I have also exactly the same problem (different color previously and now).
can you help me by telling me if there was any problem in the cell growth in culture done using this medium (faint red color medium)?
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