Is it a common event to get negative concentrations in elisa? How can I go about this especially for my controls?

06 June 2017 1 8K Report

am running an assay of activin a in urine. Could the problem be with my dilution factor?

Hi Brian, could you explain more about your assay?

There is a well known phenomenon in ELISA called the "edge effect" in which the outermost wells in the ELISA plates show different values than other wells.

Is this what you are seeing?

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