Hi,
I am wondering how to store neutravidin aliquotes. Will the protein be stable after freezing it at -20C and thawing a week later?
Thank you,
Monika
Adam B Shapiro
Do not use phosphate buffer for storing your protein at -20C. Phosphate undergoes a substantial pH change during freezing to that temperature that could be harmful to some proteins.
https://www.ncbi.nlm.nih.gov/pubmed/20039442
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Steingrimur Stefansson
Hi Monica, neutravidin is very stable in water or PBS. If you can store it at -80oC then your great-great-grandchildren will still be able to use it.
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