Can I make aliquotes of neutravidin diluted in water and PBS at -20C? If so, for how long?

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Adam B Shapiro

Do not use phosphate buffer for storing your protein at -20C. Phosphate undergoes a substantial pH change during freezing to that temperature that could be harmful to some proteins.

https://www.ncbi.nlm.nih.gov/pubmed/20039442

Steingrimur Stefansson

Hi Monica, neutravidin is very stable in water or PBS. If you can store it at -80oC then your great-great-grandchildren will still be able to use it.

Monika Makurath

Sounds great! Thank you.