An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and... | Contact experts in Antibodies to get answers
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Questions related to Antibodies
When using small chemical molecules to induce stem cells, how to screen the final small molecule combination and culture time? Including the screening of small molecule concentration, etc., please...
27 July 2023 8,217 0 View
Hi Despite considering the suitable pH of the target antigen for conjugation with the help of NAOH, after adding the target antigen to PH-adjusted gold solution, and after adding 1% BSA, and...
26 July 2023 2,583 2 View
I tried earlier with 5% Natural Goat Serum but I see a lot of background fluorescence in cells stained with secondary antibody only. I spoke with a few company represenatatives apparently they...
26 July 2023 1,309 9 View
26 July 2023 3,760 3 View
I saw some other people presenting similar problems. For a few years now, we were using Dynabeads M280, coupling them to a biotinylated antibody to perform immunopurification of target molecules....
24 July 2023 5,048 4 View
does serum starvation need to be done in the exponential growth cells? or is it okay to do it 20hr after seeding? my cells is a slow growth type cells that has ~48 hours doubling time
21 July 2023 2,271 0 View
Dear All, I am working on Rapid test development (Lateral flow based). I am facing conjugate release issue after few months of strip preparation. Up to approx 30 days, conjugates release found...
21 July 2023 8,641 8 View
I did the subcellular fractionation of transfected HEK-293T cells. For western blot analysis for the purity of fractions, I used anti-B actin antibody, anti-fibrillarin G4 antibody and...
19 July 2023 1,260 2 View
Hi everyone. I have searched all around the internet and literature for the answer to this question but haven’t been able to find any info regarding my specific situation. I have multiple...
16 July 2023 7,122 3 View
Hello! For the last 2 years, I have been producing antibodies in 293 Freestyle cells for my PhD project, and tested them against cancer cells, by verfying, first of all, binding to such cells....
15 July 2023 7,728 3 View
Hi everyone, I am about to work with gene silencing using plasmid shRNA that I bought from Santa Cruz Biotech. I am planning to do preliminary testing on different concentration of transfection...
12 July 2023 5,900 2 View
Hello all, I am seeking assistance from individuals with experience in Abaqus for a problem I'm encountering. I am currently working on the development of a buckling analysis for a honeycomb unit...
07 July 2023 450 1 View
I mean that you have it already in early childhood or not. This is shown by several of my clients who were able to get out of the parental messes in spite of enormous problems. And if it is...
07 July 2023 9,704 3 View
I'm optimising a new RNA extraction protocol from yeast. The RNA extraction with formamide works great (the yield of RNA mass/YDM is even better than our established protocol) but the final volume...
05 July 2023 7,445 2 View
Hi, I am carrying out the MTT assay using BJ-5ta fibroblasts cell line to evaluate the cytotoxicity of the nanocomposite hydrogels. I want guidance about deciding the controls in the...
05 July 2023 9,130 4 View
I want to check the cytotoxicity of my material. I am using MTT assay kit. The protocol is provided in the booklet with kit how to perform the assay. Is it better to follow the protocol provided...
04 July 2023 2,792 4 View
I used to activate my PVDF with methanol for 5 minutes, then I did transfer as usual. But these days twice I got the same results, which is there is a block white marks in between after I finished...
03 July 2023 3,280 5 View
Gromacs, protein ligand simulation
30 June 2023 4,305 3 View
I am currently trying to express Turritopsis dhornii TET enzyme with a 6x HIS tag in a BL21 E.coli host. Every time I purify my protein it shows double bands on SDS-PAGE, when testing its activity...
28 June 2023 7,857 5 View
In a wound healing experiment, I have multiple layers of cells in the sheet of cells. Only the cells in the lowermost layer of this cell sheet possess lamellipodium, while those on a higher plane...
26 June 2023 1,440 0 View
Hello, recently I’ve transfected some HEK-293T cells with Thermofisher’s Lipofectamine 3000 reagent, but I’ve only gotten a transfection efficiency of about 20-30% using 7.5 uL of lipofectamine. I...
24 June 2023 4,961 1 View
Dear colleagues! We are trying to isolate mitochondria from conifer plants. The regular Percoll step gradient isn't working due to the percularities of the material, so we want to try mixed...
23 June 2023 1,940 0 View
I am preparing polymeric water in oil (w/o)emulsion where I am using Span-80 as a surfactant. In several papers, it's given that first the emulsion has to be heated around 50-60 degree C followed...
22 June 2023 7,810 1 View
I am biotinylating a monoclonal antibody and then labeling biotinylated antibodies with gold nanoparticles (40 nm). And I will add BSA when I conjugate biotinylated antibodies with gold...
18 June 2023 4,384 1 View