An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and... | Contact experts in Antibodies to get answers
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Questions related to Antibodies
Hello everyone, I’m working on developing hippocampal and cortical cultures (Mouse pups P0-P1) with about 80–95% neuronal purity, but my results haven’t been very successful so far. I’ve been...
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For subcutaneous tumor formation experiments, which one do you recommend between Basement Membrane Matrix (Phenol Red) (HY-K6001) and Basement Membrane Matrix (HY-K6002)?
last month 5,723 1 View
I want to use the H2DCFDA probe to detect changes in ROS levels in cells after viral infection. However, since the virus cannot be taken out of the high-level laboratory for flow cytometry, I...
last month 4,952 1 View
I am interested to do id on microalgae that lnhabit surface of silt, sand,and clay in marine ecosystem. Could anybody recommend me some journals/e-book whose method works with such condition? TIA
last month 7,344 2 View
I am planning to perform animal experiments using Biochanin A with intraperitoneal administration. The required working concentration is 5 mg/mL. Could anyone share an appropriate dissolution or...
last month 8,752 2 View
I'd like to use a recombinant protein to activate B cells, but I'm unsure which one to choose. If anyone has relevant experimental experience to share, I'd be very grateful!
last month 7,868 1 View
Hi everyone, I am doing an experiment involving MSAB (HY-120697) and according to the datasheet, MSAB dissolves well in DMSO at 10 mg/ml (1 ml DMSO stock). For i.p. injection in mice (~30 g, ≤15...
last month 672 1 View
I harvested fibroblasts from an adult 3 mm punch biopsy 10 days ago. I am culturing them at 37C 5% CO2 and changing medium every 2 day (DMEM, 10% FBS, 1X Pen/Strep). Here is their appearance...
last month 250 7 View
The area of concentrations are: Green chemistry/ environmental sustainability, physical/environmental chemistry. Can I have contributions to chapters of books in the above areas of chemistry?
30 August 2025 3,363 1 View
i prefer recently articles
28 August 2025 7,370 0 View
I am modelling the modal transient response of a thin cylindrical shell using both ABAQUS and Hypermesh/MSC Nastran. Both models have the same geometry, mesh size, transient input, mass properties...
27 August 2025 5,848 2 View
I am working on CX3CL1 after induction with IPTG at 16 degrees for 24 hours and 37 degrees for 4-5 hours. I can see a band on my SDS gel both in the supernatant and pellet. When I do cell lysis...
27 August 2025 5,225 4 View
While the GAPDH ct value in 2D Panc1 cells is 21, the GAPDH ct value in Panc1 spheroids is 13. Is this result correct? If not, what could be the reason for such a result? I would be very grateful...
24 August 2025 8,629 2 View
I am trying to isolate PBMCs for an experiment and I want to remove as many platelets as I possibly can. Has anyone tried centrifuging the blood before doing the Ficoll density gradient step in...
13 August 2025 8,656 2 View
What is your opinion on this?
08 August 2025 1,753 0 View
Dear ResearchGate colleagues, I’m in the early stages of launching a new peer-reviewed scientific journal and would greatly appreciate your input and suggestions on selecting the most fitting...
03 August 2025 4,746 7 View
Hello everyone, I used the NLRP3 Antibody (YA266, HY-P80246) to detect NLRP3 protein in Ishikawa and HEC-1-A cell lines via western blot, but I wasn’t able to detect any specific bands. Does this...
31 July 2025 2,928 1 View
The expected size of the wild-type (WT) plasmid is 9.6 kb (Vector = 5.1 kb, Insert = 4.5 kb). I did a miniprep using the QIAGEN miniprep kit, and resolved 250ng of plasmid DNA on a 0.8% TAE...
29 July 2025 1,352 1 View
Are there any fixation, staining, and separation protocols for cells cultured in Matrigel? It would be best if it is for Matrigel of different thicknesses. [Questions are selected from...
28 July 2025 5,761 1 View
MTT assay
28 July 2025 1,092 4 View
So, the case is my team just finished gel extraction, and we want to know the DNA concentrations. We use EB from Geneaid for solvent, and we also use it for the blank in Nanodrop.
26 July 2025 6,866 4 View
Hi everyone, I recently performed Laurdan staining on live cells using a 10 µM working solution diluted in complete culture medium using Laurden from MCE (HY-D0080). I incubated the cells at 37°C...
25 July 2025 7,253 1 View
The 16th World Congress of Regenerative Medicine & Stem Cell-2026 (RMSC-2026) Time: July 22-24, 2026 (Next Year) Place: Helsinki, Finland Dear Corrado Mollica, I apologize for any...
24 July 2025 1,199 2 View
Hello everyone, I have been culturing mouse colon organoids on and off for over a year now. We use Matrigel domes with Stemcell IntestiCult medium. I normally split them after 9–10 days and change...
21 July 2025 6,010 6 View