An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and... | Contact experts in Antibodies to get answers
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Questions related to Antibodies
Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...
07 June 2024 7,721 1 View
Dear Researchers, I am using the classical Muskhelishvili method to solve plane stress problems in perforated plates with cracks. By introducing a conformal mapping function, the hole boundary is...
04 June 2024 3,139 0 View
Hi, I want to analyze the protein expression levels of HPV16 E6/E7 in the cervical cancer cell lines SiHa and CaSki after treatment with silencing RNA for this oncogene. Can anyone recommend a...
04 June 2024 9,538 3 View
Having the thicknesses of the layer and the substrate Having the thermal diffusivity values of coated and uncoated samples
03 June 2024 5,828 1 View
Regarding CAR-T cell therapy, during co-cultures with different effector:target ratios, when we refer to "effector", does it involve the total T cells? For example, does a ratio of 5:1 mean 5...
29 May 2024 2,709 4 View
Hello, I had a question about GVHD. We wanted to investigate the effect of a cytokine in GVHD, but we don't have the possibility to use a GVHD mouse model. Can we do it by using stimulated...
29 May 2024 8,262 1 View
I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...
28 May 2024 7,987 1 View
From CPCB website, its very confusing to get the data. Kindly provide its link.
27 May 2024 3,138 0 View
Why is agriculture diversification essential for sustainable and why immense need for crop diversification and role of agroforestry in environmental sustainability?
26 May 2024 1,846 5 View
About deficiency of calcium
25 May 2024 9,217 2 View
There is a problem of constructing a PINN based on an integro-differential equation in Tensorflow. Its simplified form is shown on the picture. The function h has no analytical solution. The...
23 May 2024 6,826 4 View
Dear Colleagues, I am currently working on a project that involves differentiating human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs)....
20 May 2024 3,116 2 View
As the title states, I am looking for a housekeeping protein for serum (for Western blots). This research is in the context of pregnancy so it has to take into account normal vs diseased (e.g....
16 May 2024 9,175 0 View
I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...
15 May 2024 7,131 3 View
Comprehensive Review of Metformin: Indications, Contraindica... Can you discuss some common adverse effects associated with metformin use?
13 May 2024 2,673 2 View
Hello everyone, hope you are doing well. I have FFPE brain arteriovenous malformation (AVM) tissues I have been trying to stain with cleaved-caspase-3. I have used the Asp175 (D3E9) CC3 9579 from...
12 May 2024 1,658 2 View
What is spatio-temporal prediction and role of GIS in spatial analysis and how is spatial analysis used in environmental management?
11 May 2024 7,068 4 View
Artificial Intelligence
10 May 2024 7,935 2 View
Dear community, I want to perform an analysis of the risilin localization within the legs of myriapods and would like to visualize the localization of the resilin components in different...
08 May 2024 9,115 3 View
Hello! I am looking to study liquid-liquid equilibrium of palmitic acid in heptane-DMSO and heptane-acetonitrile. Palmitic acid is a solid at room temperature. How would I dissolve it in my...
08 May 2024 7,548 0 View
I know that is not a transfer problem because when I use precasted gradient gel the transfer is complete. Once I use Homemade gel I have a loss in LW molecular proteins (see attachment). During...
08 May 2024 7,542 0 View
Dear community, I am currently working on a proposal focusing on cancer-associated fibroblasts (CAFs) from malignant pleural mesothelioma (MPM). In my search among commercial cell line providers,...
06 May 2024 5,529 4 View
I'm trying to gather a sample of 25 articles that span over 25 weeks from a newspaper. That means that I'm going at the rate of 1 article per week. What is the best way to sample my articles? Do I...
05 May 2024 9,586 4 View
How can we get maximum number in which country and used it. Chemical formula of it C10 H16 O. My research paper and i shall try to find out new chemical reaction which is needful.
03 May 2024 9,434 0 View