Hello Sangramjit Basak,

Add 100ul of 0.5mg/ml of MTT solution per well in a 96-well plate after removal of media for sufficient cell coverage.

Best,

Thanks Malcolm Nobre, copy that.

why do you need to remove the media?

if you want to harvest it, I would add need media or at least something to get a volume to resuspend the crystals in.

Sangramjit Basak , it is generally recommended to add about 20-50 μL of MTT solution to each well. A more common solution is 20 μL of 5 mg/mL MTT solution per well (usually the volume of a 96-well plate is about 100-200 μL, and adding 20 μL of MTT solution can cover the bottom of the cells). Then continue incubation for 4 hours. This ensures that the MTT evenly covers the cells and the reaction is sufficient.

@100 μL MTT solution is too much!

The answer to this question comes from MedChemExpress (MCE) Technical Support.

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