An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and... | Contact experts in Antibodies to get answers
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Questions related to Antibodies
i am doing THP-1 suspension culture and using cefotaxim as a antibiotic but after 4 passag my cell count was decline, so is it becuase cefotaxim my cell is decline
02 May 2024 9,757 3 View
I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...
29 April 2024 3,835 1 View
How does uneven heating of the Earth lead to wind currents and how does uneven heating of land and water on the Earth bring rainfall?
29 April 2024 6,256 5 View
Dear Researchers, The topic of my research is Investigating youths perception and attitudes toward social entrepreneurship in Wewak Urban LLG East Sepik Province Papua New Guinea. I conducted an...
25 April 2024 5,209 2 View
Can you provide examples of Level I evidence and explain why it is considered the strongest form of evidence?Understanding Evidence Levels in Evidence-Based Medicine: A ...
25 April 2024 4,388 1 View
I am trying to perform Tunel assay for senescence. DNA fragmentation is one of the characteristics in senescence. I had extracted DNA from scenescent cells and performed Tunel assay. But the RFU...
23 April 2024 4,034 2 View
Dear colleagues, I will measure serum Ang 1, Ang 2 and VCAM-1 levels by ELISA in patients who were undergone hematopoietic stem cell transplantation. I should find the most suitable dilutions...
22 April 2024 5,201 1 View
I can't find the DNA band of degenerate primer, I've done gradient PCR, DNA concentration variation, and touchdown PCR. The DNA I used is plant DNA. There is no problem with my DNA, because I have...
21 April 2024 7,442 2 View
I am working on termites and doing in situ hybridization but at the end, I can't see cells in specimen slides. my question is how to maintain the structural integrity during fixation or whole...
19 April 2024 261 0 View
Hi there, I am using 0.4% trypan blue dye to count my nuclei suspension (generated from snap-frozen intestinal colon tissue). Although I expected a blue stain only (since nuclei resemble the dead...
18 April 2024 4,456 0 View
I am Nicholas from the University of Malaya. I have been performing RNA extraction from whole zebrafish larvae for some time using NucleoZOL as my lysis buffer and passing my samples through...
17 April 2024 3,560 0 View
I have been growing HeLa to 90-100% confluency in 12 well dish, prior to subjecting them to heat stress at 43 degrees Celsius for 1.5hrs in a water bath. Unlike HEK293T, the HeLa cells lose...
17 April 2024 7,308 0 View
Hello. TetraHis antibodies recognise 4xHis-tag in the protein. What if I have a long His-tag (e.g. 10xHis-tag) and I perform ELISA or Western Blot based on native PAGE? Is there a chance that two...
16 April 2024 6,942 1 View
An imblance between the blood supply and demand could lead to failure in blood transfusion services in any country. Especially in cases where mortality and morbidity may cause to worsen in...
12 April 2024 5,294 0 View
This question is based on research done by Kavulavu et al. et al. in 2022, entitled "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya." In this...
11 April 2024 9,767 0 View
Outline the anaesthetic management of obstetric complications, placental abruption? Placental abruption is a serious obstetric complication characterized by the premature separation of the...
10 April 2024 9,342 1 View
Describe strategies for managing ethical dilemmas and navigating complex decision-making scenarios in obstetric anaesthesia care. Managing ethical dilemmas and navigating complex decision-making...
10 April 2024 9,195 4 View
This question is based on the paper “Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya” by Kavuvalu, B.M., Muendo N.N., Kumotia, W.O., Kipkulei,...
10 April 2024 5,436 1 View
This question is based on the study conducted by Kavulavu et. al. in 2022 entitled "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya". In this...
10 April 2024 197 2 View
Hi all, I am using HA beads to do a Co-IP (using drosophila whole body), then do mass spec to detect the interacting partners that pulled down by HA. The one that I tried before was not good, it...
10 April 2024 2,357 0 View
I wish to perform transwell migration assay for checking the effect of a drug on the migratory properties of Triple Negative Breast Cancer cell line (MDA MB 231). Would a difference in FBS...
07 April 2024 8,665 2 View
I have used DMSO to treat films with DMSO vapour. Can I use that same DMSO solution multiple times again?
05 April 2024 5,861 2 View
Is it like MNPT (geometrical mean of at least 20 healthy subjects) ? or it's the arythmetical mean ? can a pool of healthy subjects' plasma be considered as the reference APTT for calculating APTT...
02 April 2024 1,824 0 View
Please provide an answer along with the reference.
27 March 2024 7,460 2 View