An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and... | Contact experts in Antibodies to get answers
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Questions related to Antibodies
Hi All, I'm wondering if anyone knows where I could purchase a physical copy of the "Journal of Comparative Neurology: Research in Systems Neuroscience, Volume 524, No. 16 (November, 2016)"....
07 December 2023 2,453 1 View
I mixed EDTA mouse plasma with standard gel sample buffer and got very smeary image on Western with no distinct bands. Is there something in the plasma that needs to be removed before I run gels?
07 December 2023 8,950 1 View
Hi, I plan to conduct detailed research on the endophytic microbiome in one of Apiaceae members. I will collect root, leaf and seed samples to study endophytic microbiome. I will also analyse soil...
06 December 2023 5,045 0 View
Hello Researchers! I have a question for you. Still debating... We work with human cells. Is it OK after harvesting cells to resuspend them in Fetal Bovine Serum for further counting? Will the...
06 December 2023 5,707 1 View
We were making up the buffers for our Promega PureYield plasmid midi-prepkit and accidentally added 315ml of isopranolol to the endotoxin wash buffer instead of 210ml. Do you think we can use it?
05 December 2023 9,282 1 View
According to ancient Greek writers many people indigenous to the mountainous Cilicia province of Asia Minor had no knee-cap (επιγονατιδες, in Greek). As a result of this they were able to run at...
05 December 2023 6,895 6 View
We are using samples of the prefrontal cortex and hippocampus of mouse brain tissue. For protein extraction, we want to obtain a nuclear fraction, cytosolic fraction, and a synaptosomal fraction....
05 December 2023 1,305 0 View
How to detect soil microplastics?
05 December 2023 9,809 1 View
I am working with camel brucellosis that firstly screened using RBT(20Ag: 50 Ab) and then confirmed by cELISA using SAVANOVIR kit. To exclude prozone phenomenon, I tried to make less Antibodies...
04 December 2023 1,558 3 View
I have collected mouse fecal matter and stored at minus 80 degree C. I want to culture the gut microbiome in fecal matter and then will do MALDI-TOF MS for speciese and strain identification. Can...
04 December 2023 9,736 6 View
I want to test the efficacy of a custom made EGFR antibody via ELISA. Can anyone suggest me the best way to do it. Thanks Nidhi
04 December 2023 8,973 2 View
Hi， I am panning a anti-Human serum albumin phage library with thermo dynabeads M280 strepavidin, I can not use the popular BSA as blocking buffer any more, so any suggestion? Caisin has biotin,...
03 December 2023 168 0 View
Research or review
03 December 2023 7,083 3 View
How to check pH of PCR mastermix?
01 December 2023 1,392 3 View
I have recently started working with the Insitu hybridisation for Cd31 in P0 mice in cryosections of 10um. I don’t see any signal . but worked for Embryonic stage ! I dont know how to change the...
01 December 2023 1,597 1 View
Can anyone suggest why the D283 medulloblastoma cell line did not attach after thawing? What changes can be made to the dish surface or culture medium to solve this?
01 December 2023 3,296 2 View
I am performing qRT-PCR for zebrafish different organs and tissues using sybergreen and elongation factor 1-alpha elfa as a reference control. However, there is variation in the expression levels...
30 November 2023 9,284 2 View
I want to know more about ultrapotassic magmatic rocks.
30 November 2023 5,598 1 View
There's a great app called ChemSketch but only compatible with Windows. I'm looking for similar options for a Mac user. Thnks!
30 November 2023 3,213 2 View
I prepared GST-tagged protein. The molecular weight of my protein (50kda) with GST (26 kDa) tag is 76 kDa. After removal of GST tag the molecular weight should be 50 kDa but in SDS page I got 40...
29 November 2023 6,184 3 View
I was doing transformation using plasmid with kanamycin resistance into BL21 DE3 E. coli cells with ampicillin resistance. I plated the culture on amp+kana plates and amp only plates. However,...
29 November 2023 7,936 3 View
The low temperature tolerance of high-latitude species is greater than that of low-latitude species, while the high temperature tolerance of high-latitude species is less than that of low-latitude...
28 November 2023 6,596 0 View
In research where the objective is to study the impact of microplastics on soil properties, is it necessary to evaluate the original microplastic concentration of the test soils? If the soils...
28 November 2023 215 8 View
Hey Researchers, i am working with transformation of membrane protein by using BL21(DE) competent cell. i transformed plasmid (pET28a(FLAG, His tag) and co-transformed with pULTRA-ambryx. i...
27 November 2023 3,571 0 View