An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and... | Contact experts in Antibodies to get answers
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Questions related to Antibodies
For flow cytometric analysis of N1 and N2 phenotype of TANs in breast tumor mice, I need to specifically identify them for ordering antibody and is unable to confirm the correct one. If anybody...
10 January 2024 870 0 View
I am running SDS page gels using the mini-PROTEAN tetra vertical tank. Gels ran normally up until about 3/4 of the way down the gel. At this point, the dye changes from blue to yellow and became...
10 January 2024 7,273 3 View
I need to evaluate abdominal adipose tissue hypertrophy in a murine model with metabolic syndrome, with and without treatment and I need to review which is the best objective to measure adipocyte...
10 January 2024 7,132 0 View
How do matter and energy move through Earth's oceans and atmosphere and how is matter and energy transferred by convection within Earth's layers?
10 January 2024 2,441 4 View
RG citation score is frozen. Anyone noticed that with his/her profile?
10 January 2024 4,883 2 View
Hello! The freezing protocol for MSCs I have been performing says to freeze 1 ml of cell suspension. I was told that in order to keep confluence, I must add only 1 ml to the pellet of cells and...
09 January 2024 1,746 2 View
Cellular biology of human
08 January 2024 6,520 4 View
if cane answer it
08 January 2024 1,293 0 View
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07 January 2024 5,681 1 View
I did an experiment for a positive control phos-tag system to detect phosphorylated AMPK in min6 cells. I incubated min6 cells for 12h with metformin to induce AMPK phosphorylation then check the...
07 January 2024 7,726 1 View
Can I change an uploaded file from "adding a public file" to "adding a private file" in ResearchGate?
07 January 2024 8,450 1 View
Greetings all! I am seeking help with a question I recently stuck with. In the images below, you can see an example of the immunostaining of brain tissue. There is only DAPI and auto-fluorescence...
05 January 2024 5,137 10 View
I am looking for TP53 status of various carcinoma cell line from DepMap portal (https://depmap.org/portal/gene/TP53?tab=characterization&characterization=mutation), but could not get the P53...
04 January 2024 1,619 0 View
Guys, good night. I need to use a comparative genomics tool that demonstrates synteny between three species of a genus. But I would like something more visual. I've already used mauve, I don't...
04 January 2024 579 3 View
Has anyone tried this? I want to lyse whole blood samples, but I'm not finding a protocol for it using RIPA buffer. I found a recipe for a lysing buffer, but it uses different salts (which I don't...
04 January 2024 649 0 View
I am looking to get a placental cell line that Fibronectin FN1 endogenously expresses. My cell line choices are HTR-8/SVneo and JEG-3, but when I checked the FN1 expression in these cell lines...
04 January 2024 5,337 0 View
Human genetic makeup in detail
03 January 2024 5,755 2 View
03 January 2024 3,071 6 View
What is the role of microorganisms in reducing soil pollution and relationship between microorganisms in soil and plants?
03 January 2024 2,722 4 View
I am searching for antibody to rat brain tissue. I would prefer to purchase antibody for both Western blot and Immunohistochemistry. Thank you in advance.
02 January 2024 6,312 1 View
Hello all, I am trying overlapping mutation for a site specific point mutation ( I tried with QuickChange kit, not working) . For which I have to first generate two fragments of my target gene....
30 December 2023 8,762 3 View
How to prepare the sections and how to immunostain with three different antibodies? As I am going to use antibody to stain receptor of interest and anti insulin and antiglucagon. How can the three...
29 December 2023 7,135 0 View
Hi I am doing experiments with Rotenone-induced SH-SY5Y for PD research. I have seen some papers mentioning PD on their introduction but their methods with rotenone-induced SH-SY5Y do not...
29 December 2023 5,243 3 View
In a set of bacterial proteomics results from the Koala software I have numerous protein records (many with Protein Score > 100, and with simultaneously high (i.e., >1) emPAI, +-high Match....
27 December 2023 325 2 View