Hello everyone, I’m working on developing hippocampal and cortical cultures (Mouse pups P0-P1) with about 80–95% neuronal purity, but my results haven’t been very successful so far. I’ve been using Ara-C at 1 µg/ml for a 12-hour incubation, but the outcome still doesn’t look good. Could anyone share if their lab has already optimized a protocol, and suggest which antimitotic agent works better for achieving higher neuronal purity (Ara-C or FUDR)?