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Questions related from V H Krishna Prasad
Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...
24 June 2024 8,741 5 View
Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...
05 June 2024 1,459 0 View
Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...
18 April 2024 8,473 3 View
Hey all I wish to know the change in expression level of certain genes post treatment with drug. For this can I isolate RNA after 24hrs of treatment? Should I wait till 48 hours? Or should I...
07 April 2024 9,040 0 View
Hey all Suppose the yield of nanoparticle synthesized is 20mg. How much of this should be dissolved in appropriate solvent and how much of solvent should this be dissolved in for Zeta and DLS...
05 April 2024 8,432 6 View
Hey all I had dissolved a hydrophobic drug in DMSO and kept it in 4 degrees. The storage condition mentioned was 2-8 degrees. It had become ice in that temperature. What is most appropriate...
16 March 2024 468 0 View
For transfection we have used 500uL of transfection complex for 1 well of 6 well plate. so total media added to each well was 2mL. According to this 100nM of sirNA and 100ug/mL of nanoparticle was...
11 March 2024 4,956 2 View
How is DLS and zeta potential of nanoparticle carried out? How to know how much dilution of nanoparticle to make for zeta potential analysis?
23 February 2024 993 1 View
Hey all A few of my lab-mates store the protein after quantification (I used bradford assay) in cell lysis buffer containing protease inhibitor in -80. While others add loading dye and denature...
24 December 2023 9,803 4 View
Hey all I want to see the affect of a certain drug on my cell line (cancer cell line). I want to see how it alters the expression of a certain nuclear protein. Since my cells are fast growing...
19 December 2023 4,688 2 View
Hey all I have seeded cancer cells in 6 well plate and intend to check the expression of certain protein with and without treatment with the drug at our disposal. The cells have adhered and...
27 November 2023 7,572 4 View
Hey all I followed the below attached protocol for synthesis of Chitosan nanoparticle. However after 12 hours of continuous lyophilisation I didn't get it in the powdered form. How does chitosan...
15 October 2023 8,145 2 View
Hey all I tried reviving the HaCaT cell line using the standard protocol for cell revival. However, they aren't attached to the flask even after 72 hours. The cell lines were stored in August...
30 August 2023 7,693 3 View
Hey all I have been trying to plot the survival fraction (SF) data obtained from the clonogenic assay using GraphPad Prism Version 5. I don't have access to the latest version. What graph type...
22 August 2023 2,138 3 View
Hey all Usually, at what confluency is a wound healing assay performed? I have cells below 70-80% confluency in a 6-well plate. Is this stage enough to do the assay?
09 August 2023 8,922 5 View
I seeded two flasks (T-12.5) in two different CO2 incubators. They contained the same number of cells, the same media, etc. The one-seeded in the rarely used incubator had an abnormal morphology (...
26 June 2023 4,996 10 View
Hey all I have lyophilized chitosan nanoparticles, and I got a sticky kind of substance instead of a powder. Is the problem because of a fault in the sonication process that failed to become...
13 June 2023 3,198 5 View
I am hoping to be proven wrong. Kindly go through the attachment and provide me with your thoughts on this
29 April 2023 5,166 4 View
Hey all Does chitosan dissolve properly in 1% acetic acid or is it a colloidal solution? I have attached an image for clarity and the source from where I got the protocol....
24 April 2023 2,877 4 View
Hey all I recently revived a vial of adherent oral cancer cells and plated them into a T-75 flask. I could notice so many dead/non-adherent cells floating in the media. I thought this would stop...
07 April 2023 7,127 3 View
Since we check the ability of cells to form a colony in colony formation assay, must they be plated with solution containing complete media or just media with drug Thank and best
02 March 2023 137 2 View
Hey all Let me know if it is possible synthesis a protein nanoparticle in the form of micelle encapsulating a hydrophobic drug inside the core
20 February 2023 8,472 0 View
Hey all I know that it has something to do with the absorbance, however the concept is not so clear to me. Kindly elaborate or provide a link
08 February 2023 9,106 4 View
Hey all, I have been asked to write a review paper. I have went though many papers and now I'm a bit unsure as to which article to site while writing the paper. Should I go for the recent reviews...
07 February 2023 6,805 5 View
Hey all We plan to treat oral cancer cell lines with the drug piperlongumine, which happens to be hydrophobic in nature for MTT assay. Let us know usually in which solvent hydropohic drugs are...
01 February 2023 5,851 3 View
hey all I have seeded some cells for colony assay in a 6 well plate. I intent to fix it and stain it with crystal violet. (0.5%). I don't have formalin, can I use 70% ethanol as fixative?...
30 December 2022 3,169 3 View
I have been trying to do Clonogenic assay for a while now. It has been unsucessful so far standardizing the right number of cells to form colony in a 6 well plate. Kindly guide me in preparing this
27 December 2022 4,401 1 View
Hey all I autoclaved a bunch of 50 and 15 ml falcon tubes (Tarson). I wish to use those 50ml falcon tubes for preparing and storing complete media. Am i good to go ahead with this? or should I...
09 December 2022 870 2 View
Hey all I noticed that when I forgot to passage HeLa cells after 10 days the media colour had changed to yellow and I could see colonies everywhere. I gave multiple 1X PBS wash and passaged them....
03 December 2022 2,075 4 View
Hey Guys I noticed a change in FBS's colour from slightly yellow to brown after I placed it in a water bath at 37 degrees C for 5 minutes. Any reason why this happened?
29 November 2022 3,960 0 View
Does the ethanol used in labs stay pure forever? I just opened a bottle of sealed ethanol that was purchased in 2015 and it kind of smells different. Does ethanol expire after certain time of storage?
23 November 2022 4,769 4 View
We do not have UV light inside our incubator. In such situations how to sterilize the insides of an incubator which was kept non functional for long? I have gently scrubbed the inside with...
22 November 2022 7,227 5 View
Hey Guys I split HeLa cells from a confluent T25 flask into 4 T25 flak. The T-25 flask was over confluent and the media was yellow. I changed the media, gave the cells 1XPBS wash and split them...
21 November 2022 8,307 4 View
Does the lack of ions make it non consumeable, if so why?
17 November 2022 7,389 9 View
Hey Guys I have seeded HeLa cells (1000 cells per well in a 6 well plate). Is the density too low? Considering that they their doubling time is 33-26 hrs. I wanted to do Clonogenic assay Thanks...
14 November 2022 5,168 2 View
Hey Guys I am working on HeLa cells and I need to do a clonogenic assay. Following are the steps I follow for seeding 1. Remove media and wash with 1XPBS 2. Add trypsin and incubate for 1...
08 November 2022 1,120 0 View
Hey guys I need 1XPBS for cell culture work, for washing the cells and removing the dead debris. So I am a bit confused because autoclaving the 1XPBS kills almost all bacteria but I have seen...
06 November 2022 3,960 4 View
Hey Guys Which among the above mentioned is most suitable material for nanoparticle formulation and targeted drug delivery ? thank you
26 October 2022 4,219 5 View
Hey Guys I have to store cells in liquid nitrogen for long time preservation. I have 4 T-75 flasks with 80% confluency. Should I take a vial for each flask? or should I make it concentrated by...
15 October 2022 6,409 0 View
After splitting I noticed these yellow climbs floating in the media, are these dead cells or contaminated? The media color appears to be the same as before 2 days.
11 October 2022 972 2 View
I transferred KB cells stored in -80 into a T-75 flask by adding 10% FBS media without centrifugation. I plan to change the media after proper adhesion of the cells. I don't know how many days it...
05 October 2022 999 3 View
Is 1500rpm for 5-10 minutes not sufficient to pellet down HeLa cells? Why couldn't I see any pellet? Please answer
02 October 2022 7,927 5 View
To check for contamination I put DMEM media alone in a culture dish followed by incubation at 37C and 5% CO2. After 24hrs I the media was of same color, I could observe these in the microscope....
02 October 2022 5,605 0 View
Hey all Most scientists and Scholars avoid using female mice in research because of estrous cycle. However there are many publications where exclusively females are selected for the procedure....
01 January 1970 9,470 6 View
Monocytes get transformed into macrophages upon reaching tissues. But do the monocytes have any phagocytic properties?
01 January 1970 7,040 2 View
Hey Guys Often I have noticed that these two words are used with similar meaning in the Oncology domain. Do they have any difference or are the synonyms? Best
01 January 1970 6,560 0 View
Hey all Can colon cancer be induced in mice by injecting human cancer cells? While there exists many papers on using chemicals such as DMH and AOM, a very few exist where they have used cells for...
01 January 1970 9,040 0 View
Hey all I added 10uM of primer instead of 0.5uM in a 10uL PCR reaction. What are the expected results? Will the amplification of the desired product happen by any chance?
01 January 1970 598 5 View
