Hey all
Does chitosan dissolve properly in 1% acetic acid or is it a colloidal solution? I have attached an image for clarity and the source from where I got the protocol.
https://pubs.rsc.org/en/content/articlelanding/2016/ra/c6ra12699e
The following represents the protocol I followed for the synthesis of Chitosan nanoparticle.
1. Weighed 1.25g of Chitosan powder (from Sigma)
2. Made 1% (v/v) acetic acid solution in 500mL double autoclaved water.
3. Added 1-2 mL of tween 20
3. Stirred the solution at room temperature for 24hrs
But the particles are not dissolved and have formed a kind of colloidal solution with froth over the top.
If anyone out there has a standard protocol for making Chitosan nanoparticles, kindly provide it. Also please point out my mistake.
Chitosan doesnt dissolve completely above a certain limit. It may form gel like viscous solution. At present you are talking about 2.5% chitosan solution.
You can alternatively follow a simple step, of adding chitosan in small amounts to the solution. 2% acetic acid also can be used
You should also add a cross linker (tripoly-phosphate and glutaraldhyde are some common examples) in order to produce the nanoparticles. I also think that the chitosan concentration is too high for nanoparticle formation. I think you should decrease it.
Thank you Eloy Pena Rodríguez for your suggestion. I did add 1% glutaraldehyde.
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