Troubleshooting Chitosan Nanoparticle synthesis ?

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Ester cleavage conditions?

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Hortensia Ortega-Ortíz

Powders are not necessarily obtained, the important thing is that the solid you obtain is very dry.

Then you can make a dispersion of the solid and check the size of the chitosan particles by DLS.

V H Krishna Prasad

Thank you very much for your insight Hortensia Ortega-Ortíz

I added 100% ethanol and crushed the lyophylised product. I got them in powdered form. However I am yet to check the size by DLS.

If there is any method to obtain them in uniform size below 450nm, please suggest.