Hey all
I have lyophilized chitosan nanoparticles, and I got a sticky kind of substance instead of a powder. Is the problem because of a fault in the sonication process that failed to become powder?
Please have a look at the attachment
Chitosan nanoparticles dont become the way shown in image.
When lypholized they become flackish but crushable
flackish ? Henry Kolge
Sorry I did not get you. It appears crushable, though.
Dear Henry Kolge can you tell me for how many hours the lyophilisation process needs to be carried out for 0.9g of low molecular chitosan dissolved in 0.5%(v/v) acetic acid ? After 24hrs in magnetic stirrer it was passed through a membrane filter (0.45 micron) , centrifuged at 13,000 rpm for 2 hrs and solvent discarded. Following that I had initially lyophilised it for 3 hrs after which I observed a sheet like subtance. Then I placed in the lyophiliser again.
Could you please share me an image of lyophilised chitosan nanoparticle?
Also suggest the time duration of lyophilisation?
P.S. TPP was used as a cross linker
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