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Questions related from V H Krishna Prasad
Hey all Usually, at what confluency is a wound healing assay performed? I have cells below 70-80% confluency in a 6-well plate. Is this stage enough to do the assay?
09 August 2023 8,887 5 View
I seeded two flasks (T-12.5) in two different CO2 incubators. They contained the same number of cells, the same media, etc. The one-seeded in the rarely used incubator had an abnormal morphology (...
26 June 2023 4,987 10 View
I am hoping to be proven wrong. Kindly go through the attachment and provide me with your thoughts on this
29 April 2023 5,155 4 View
Hey all Does chitosan dissolve properly in 1% acetic acid or is it a colloidal solution? I have attached an image for clarity and the source from where I got the protocol....
24 April 2023 2,871 4 View
Hey all I know that it has something to do with the absorbance, however the concept is not so clear to me. Kindly elaborate or provide a link
08 February 2023 9,092 4 View
Hey all, I have been asked to write a review paper. I have went though many papers and now I'm a bit unsure as to which article to site while writing the paper. Should I go for the recent reviews...
07 February 2023 6,792 5 View
Hey all We plan to treat oral cancer cell lines with the drug piperlongumine, which happens to be hydrophobic in nature for MTT assay. Let us know usually in which solvent hydropohic drugs are...
01 February 2023 5,837 3 View
hey all I have seeded some cells for colony assay in a 6 well plate. I intent to fix it and stain it with crystal violet. (0.5%). I don't have formalin, can I use 70% ethanol as fixative?...
30 December 2022 3,163 3 View
I have been trying to do Clonogenic assay for a while now. It has been unsucessful so far standardizing the right number of cells to form colony in a 6 well plate. Kindly guide me in preparing this
27 December 2022 4,390 1 View
Hey all I autoclaved a bunch of 50 and 15 ml falcon tubes (Tarson). I wish to use those 50ml falcon tubes for preparing and storing complete media. Am i good to go ahead with this? or should I...
09 December 2022 862 2 View
Hey all I noticed that when I forgot to passage HeLa cells after 10 days the media colour had changed to yellow and I could see colonies everywhere. I gave multiple 1X PBS wash and passaged them....
03 December 2022 2,064 4 View
Does the ethanol used in labs stay pure forever? I just opened a bottle of sealed ethanol that was purchased in 2015 and it kind of smells different. Does ethanol expire after certain time of storage?
23 November 2022 4,758 4 View
Hey Guys I split HeLa cells from a confluent T25 flask into 4 T25 flak. The T-25 flask was over confluent and the media was yellow. I changed the media, gave the cells 1XPBS wash and split them...
21 November 2022 8,299 4 View
Does the lack of ions make it non consumeable, if so why?
17 November 2022 7,377 9 View
Hey Guys I have seeded HeLa cells (1000 cells per well in a 6 well plate). Is the density too low? Considering that they their doubling time is 33-26 hrs. I wanted to do Clonogenic assay Thanks...
14 November 2022 5,157 2 View
Hey Guys I am working on HeLa cells and I need to do a clonogenic assay. Following are the steps I follow for seeding 1. Remove media and wash with 1XPBS 2. Add trypsin and incubate for 1...
08 November 2022 1,061 0 View
Hey Guys Which among the above mentioned is most suitable material for nanoparticle formulation and targeted drug delivery ? thank you
26 October 2022 4,210 5 View
I transferred KB cells stored in -80 into a T-75 flask by adding 10% FBS media without centrifugation. I plan to change the media after proper adhesion of the cells. I don't know how many days it...
05 October 2022 987 3 View
Is 1500rpm for 5-10 minutes not sufficient to pellet down HeLa cells? Why couldn't I see any pellet? Please answer
02 October 2022 7,916 5 View
To check for contamination I put DMEM media alone in a culture dish followed by incubation at 37C and 5% CO2. After 24hrs I the media was of same color, I could observe these in the microscope....
02 October 2022 5,592 0 View
Hey all Most scientists and Scholars avoid using female mice in research because of estrous cycle. However there are many publications where exclusively females are selected for the procedure....
01 January 1970 9,460 6 View
Monocytes get transformed into macrophages upon reaching tissues. But do the monocytes have any phagocytic properties?
01 January 1970 7,033 2 View
Hey Guys Often I have noticed that these two words are used with similar meaning in the Oncology domain. Do they have any difference or are the synonyms? Best
01 January 1970 6,552 0 View
