Hey all, thank you very much in advance.
These are A549 lung cancer cell lines. While I had received the flask from a friend of mine, they appeared good. After the second passage, they are showing this morphology. They appear to be stressed. I had changed the entire media, serum, and antibiotics, yet the problem seems to be persistent. I have attached a normal A549 morphology and the abnormal one for insights from this community.
Since you have already changed the media, serum, and antibiotics, I would suggest checking for contamination (especially mycoplasma) and reviewing your passaging technique
I agree with Maisra Azhar . You definitely want to consider treating for mycoplasma and refining your technique to avoid any chance of reintroducing it again in the future.
Thank you, Benjamin W. Elwood and Maisra Azhar I will certainly consider treating for mycoplasma and increase the concentration of antimycotics as well.
- Badges
- Science topic