49 Questions 15 Answers 0 Followers
Questions related from Ellie Major
How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?
21 March 2016 3,637 2 View
I will soon be needing to dissolve sucralfate and would like to know if isopropyl alcohol can be used instead of ethanol. I know that ethanol is what is recommended, however I would like to know...
14 March 2016 5,070 2 View
Is chondroitin sulfate a "sulfated moni, di, or oligosaccharide"? If not, can you please name something or a list of substances that are "sulfated mono, di or oligosaccharide"? Thank you.
27 February 2016 4,292 1 View
If I have a powdered or ground plant, how can I make a petroleum ether extract of it?
14 April 2015 2,155 15 View
In a hypothetical experiment, do you think that having a patient soak their scalp in a solution suspected to have hair growth promoting effects would be more beneficial than simply applying the...
13 March 2015 7,419 4 View
I am sure this question is going to be one of the more peculiar ones ever asked here, so please forgive the strange atmosphere of the question! Being that I own my own laboratory and I wanted to...
17 February 2015 189 7 View
I am hoping to find somebody who might know the gossypol content of cottonseed oil. Is gossypol available in high quantities in cottonseed oil? If not, where might I find an abundance of gossypol?
14 February 2015 8,634 5 View
What would be the best way (or how you would personally go about doing this) to make a liquid solution using a powdered plant extract without subtracting from the plants natural medicinal effects...
10 February 2015 5,164 18 View
Is there a substance that if applied transdermally, would induce igf-1 proliferation or release?
06 February 2015 7,513 3 View
Hello everyone, I am hoping to have qualified researchers help settle the debate regarding the safety of aspartame and to help separate fact from fiction. Typically, the only people you can find...
25 January 2015 6,325 6 View
What growth factors are in urine and how do you isolate them? Any VEGF, FGF, PDGF?
20 January 2015 8,383 2 View
What substances can platelet derived growth factor be found abundantly in? Excluding blood, prp, cord blood (any blood fractions or types), bone marrow, where can I find large quantities of pdgf?...
20 January 2015 8,811 2 View
Hello, Hopefully, someone can please help me out with my misunderstandings surrounding prp. I have been told that the standard protocol for prp Iisolation from whole blood is 160xG for 15 minutes....
29 December 2014 8,563 9 View
Hello, I would like to know what the risks and possible negative effects of daily electroporation of facial skin are. Are there any negative side effects that may occur if electroporation of...
21 December 2014 6,513 1 View
Hello, Does anyone know of a protocol for in vivo skin electroporation? I will be undergoing a trial where I will be applying pdgf to the skin of my patients and then using electroporation to...
10 December 2014 4,257 1 View
I will be conducting an in vivo test to define transdermal delivery via topical/skin electroporation. I will be applying a drug to the patients skin and using an in vivo electrode to carry the...
24 November 2014 8,912 8 View
I will be performing a test soon that I have been planning for awhile, though one are still hangs me up. The test will involve applying a solution to a human patients scalp, and electroporating...
17 November 2014 5,651 4 View
The specific area of fate regeneration I am referring to is the nasolabial folds. Imagine that you have a patient with a bodyfat percentage of 3% and not the typical nasolabial fold patient who...
05 November 2014 6,661 3 View
I am going to isolate prp from whole blood and I will be using the absolute basic essentials. A centrifuge, and ficol 1077. I will be using 18ccs of blood. Can someone please tell me the best...
03 November 2014 9,486 4 View
I have recently bought a drug (prostaglandin F2a) and want to make sure it is authentic. Is there a lab that offers a verification service? A lab that I could send a sample in and they would give...
20 October 2014 2,926 1 View
I will be using ethanol, proplyne glycol and water as solvents for the drug bimatoprost. Should I use denatured or undenatured? Thank you very much!
27 September 2014 3,490 2 View
I want to mix a certain chemical with an oil, however I do not know if it will be viable after it comes in contact with the oil. Can someone tell me how to determine a chemicals stability in the...
08 September 2014 6,965 3 View
Thank you for helping me. What I want to do, is use 10 ml's of ethanol with 10mg's of pge2, but I am not sure if these preparation instructions will contradict or lessen the efficacy of the final...
20 July 2014 7,229 0 View
Does anyone know how long PGE2 will stay stable in PBS? Also, how can I determine whether it will stay stable in a substance such as emu oil?
13 June 2014 4,924 0 View
I have heard that prostaglandin E2 is a semen derivative. Does that mean that it is found largely in semen? How much prostaglandin E2 is in semen?
06 June 2014 660 1 View
I have been told about 4mm. Is that true? Thanks!
04 June 2014 3,638 1 View
For those of you who don't know, there is a procedure called "SNIF", or sharp-needle-intradermal-fat graft. Here is a video for it, and a link to a pdf that details it in excellent easy detail....
02 June 2014 6,789 4 View
Should only sterile water be used when a substance says it is water soluble? I will be using PGE2 and don't want to ruin it's efficacy.
29 May 2014 6,738 2 View
I have a few questions that may seem like beginner questions, but I would really like to know what I must consider when making a solution and keeping efficacy. I will be trying to make a topical...
10 May 2014 231 1 View
From the time you inject preadipocytes intradermally, how long will adipogenesis take? Let's say you were wanting to induce adipogenesis in the scalp. You inject preadipocytes intradermally, then...
09 May 2014 1,878 1 View
I have a digital scale that reads in the format 0.00 and in grams. I need to measure 4.5 milligrams. Would I still be able to use this scale? Shouldn't it read 0.45 g for 4.5 mg's? I'd settle for...
19 March 2014 1,136 8 View
This may seem like a foolish question and I admit I am a beginner, but it still baffles me how scientists can inject such tiny volumes! For example, there is a study that does the following in a...
14 February 2014 373 5 View
I need to know how this is done. Most cannulaes are over a foot in length and won't fit in an autoclave. Is there another way to sterilize it? Povidone Iodine?
09 January 2014 504 3 View
I need someone to help me with each step assuming that I have 20ml's of adipose tissue. There's nothing wrong with this protocol, I'm just really bad at filling them in and want to get this...
24 November 2013 3,504 2 View
The following is hypothetical, but still very important. Kind of fun to think of actually. So scientists at Yale have apparently used preadipocytes isolated from a bald rat's adipose tissue to...
17 November 2013 9,300 1 View
I will need some assistance here. I need someone help me to put real numbers in place of the variables in the following protocol. You don't need to know anything about this particular method or...
15 November 2013 5,303 3 View
Before a bone marrow aspiration, how would you sterilize the needle? Not that it's a used needle, but just to be safe!
12 October 2013 7,344 1 View
I need to know how to isolate the MNC's from bone marrow. I know how up to a certain point, and I am hoping you can take a look and fill in the blanks. So I have 50ml's of marrow and am isolating...
01 July 2013 7,560 1 View
Hi, I have 50ml's of bone marrow. I would like to know how many ml's of pbs I should dilute it with, as well as how many ml's of ficoll I should use. I will be using 50ml centrifuge tubes. Also,...
18 June 2013 690 2 View
If I have about 30 milliliters of adipose tissue, how would I isolate preadipocytes from it? Also about how many cells would 30mls contain? (estimation)
11 June 2013 978 1 View
What temperature should bone marrow be stored and how long can it be exposed to room temperature conditions? As in a centrifuge tube. Should it be frozen? What should it be frozen in? I have 250...
11 June 2013 5,663 4 View
I am having difficulty understanding how I can replicate a study's success. Could you take a look at this old study (patent really)? They have done what I hope to, which is achieve a volume of...
05 June 2013 2,453 1 View
I need to know how to isolate CD133 cells from bone marrow. I will be using the isolation microbead kit from miltenyi biotec. There are a few challenges I have that I cannot seem to get a...
28 May 2013 9,397 5 View
I am still trying to find a protocol for preadipocyte isolation from adipose tissue. Can someone please tell me how to get PREadipocytes from adipose tissue?
24 April 2013 6,476 10 View
I am having a little difficulty with the basics of isolating cd133 cells from peripheral blood. My goal is to end up with 5 milliliters(or more) of cd133 cells. I am used to separating cells using...
27 March 2013 3,939 2 View
What human stem cell is most similar to embryonic stem cells(in terms of multipotency/ multipurpose)? Nothing that is derived from umbilical cord blood or bone marrow. Peripheral blood and adipose...
17 March 2013 4,185 4 View
I am desperate for help on this matter. Can anyone help me to isolate adipocyte precursor cells from adipose tissue?
12 March 2013 3,886 1 View
I am wondering which stem cells are referred to when talking about stem cells derived from adipose tissue. Anyone know? I know cd34 and cd133 positive cells are among them, but that can't be all...
07 March 2013 2,962 1 View
I am currently a student looking to get into the field of biotechnology upon graduation and am currently interning at a privately owned biotechnology facility. At said facility, there is an...
26 January 2013 2,550 7 View
