Do you know someone with knowledge of France in 1700s?

I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...

01 October 2020 9,095 5 View

How to detect gamma H2AX and cleaved caspase 3 signal for WB?

I am trying to detect any differences in DNA damage/apoptosis related proteins by western blot between wild type and disease cells. I ran an initial WB and saw no bands for either protein and am...

18 November 2019 7,204 4 View

How do you measure CO2 in Binder Incubators?

We have two Binder incubators which we'd like to check the CO2 concentration in. On our Thermo incubators we used a Fyrite Gas Analyzer but these incubators don't seem to have a port to measure...

14 November 2019 7,586 1 View

Any open access meterological data for Spiti Valley Himachal Pradesh?

Hello, I am doing some research for my dissertation and require meterological (ppt and temp) data for Spiti Valley, Himachal Pradesh. Although I have found some data for the Lahul and Spiti...

15 October 2018 7,769 4 View

How to interpret Co-Correspondence analyses (CoCA)?

I'm trying to interpret the results of Co-correspondence analysis (CoCA) with the R package cocorresp. The descriptive paper is rather unclear about how to interpret the results. I've performed...

25 February 2018 2,999 2 View

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

20 March 2016 3,437 2 View

Can isopropyl alcohol be used to dissolve sucralfate in place of ethanol?

13 March 2016 4,920 2 View

Can someone help me to calculate electrochemical surface area (ECSA) ?

14 December 2015 5,519 7 View

Can doxorubicin affect protein migration on SDS-PAGE?

I have been treating cardiomyocytes with various amounts of doxorubicin (between 250nM-2uM) and running my cell lysates (20ug protein in RIPA with protease /phosphatase/deacetylase inhibitors) on...

24 June 2015 8,712 3 View

How can I make a petroleum ether extract of a plant?

If I have a powdered or ground plant, how can I make a petroleum ether extract of it?

13 April 2015 2,009 15 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View