Do you know someone with knowledge of France in 1700s?

I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...

01 October 2020 9,095 5 View

How to detect gamma H2AX and cleaved caspase 3 signal for WB?

I am trying to detect any differences in DNA damage/apoptosis related proteins by western blot between wild type and disease cells. I ran an initial WB and saw no bands for either protein and am...

18 November 2019 7,204 4 View

How do you measure CO2 in Binder Incubators?

We have two Binder incubators which we'd like to check the CO2 concentration in. On our Thermo incubators we used a Fyrite Gas Analyzer but these incubators don't seem to have a port to measure...

14 November 2019 7,586 1 View

Any open access meterological data for Spiti Valley Himachal Pradesh?

Hello, I am doing some research for my dissertation and require meterological (ppt and temp) data for Spiti Valley, Himachal Pradesh. Although I have found some data for the Lahul and Spiti...

15 October 2018 7,769 4 View

How to interpret Co-Correspondence analyses (CoCA)?

I'm trying to interpret the results of Co-correspondence analysis (CoCA) with the R package cocorresp. The descriptive paper is rather unclear about how to interpret the results. I've performed...

25 February 2018 2,999 2 View

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

20 March 2016 3,437 2 View

Can isopropyl alcohol be used to dissolve sucralfate in place of ethanol?

13 March 2016 4,920 2 View

Can someone help me to calculate electrochemical surface area (ECSA) ?

14 December 2015 5,519 7 View

Can doxorubicin affect protein migration on SDS-PAGE?

I have been treating cardiomyocytes with various amounts of doxorubicin (between 250nM-2uM) and running my cell lysates (20ug protein in RIPA with protease /phosphatase/deacetylase inhibitors) on...

24 June 2015 8,712 3 View

How can I make a petroleum ether extract of a plant?

If I have a powdered or ground plant, how can I make a petroleum ether extract of it?

13 April 2015 2,009 15 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View