Before a bone marrow aspiration, how would you sterilize the needle? Not that it's a used needle, but just to be safe!
with 10% povidone-iodine solution (Betadine Solution)
I am looking to study if plasma cells in one of the models I am working on secrete various levels of cytokines, but I am unsure which stimulation in vitro should be used to look at this? I have...
31 July 2023 9,230 1 View
I have human cells that I've digested in proteinase K and I'm about to precipitate the glycosaminoglycans in the sample using ethanol, but as I understand it this will also precipitate any nucleic...
05 June 2023 7,000 0 View
Good day, I am currently in year 2 of my Master's. My research/dissertation topic is “How can teachers adapt their practice to deliver inclusive PE lessons to enhance student engagement?” As...
18 December 2022 4,911 1 View
I am aiming to distribute an online survey through social media with the target audience being generation z consumers that purchase in the fashion industry. I wasn't sure what type of sampling...
06 November 2022 1,265 3 View
I am attempting to access The Traumatic Stress Institute Belief Scale for my final year project, and I can not seem to able to access it anywhere. Does anyone know where I can find the actual scale?
19 January 2022 4,281 1 View
Hi there! I need to examine modifications of titin in cardiomyocyte culture. Since I won't collect enough protein if I use primary culture (needs to be adult), I was wondering if anyone has looked...
09 March 2021 4,040 0 View
I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...
01 October 2020 9,406 5 View
I am trying to detect any differences in DNA damage/apoptosis related proteins by western blot between wild type and disease cells. I ran an initial WB and saw no bands for either protein and am...
18 November 2019 7,377 4 View
We have two Binder incubators which we'd like to check the CO2 concentration in. On our Thermo incubators we used a Fyrite Gas Analyzer but these incubators don't seem to have a port to measure...
14 November 2019 7,722 1 View
Hi, I am trying to calculate the overall error of my glacier mass change calculations. I have identified the following sources of uncertainty: Volume - Area change of glacier (+/- 3%) -...
17 February 2019 824 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hi all, Second question on the same or so topic. I am working on subroutines (UMAT and others) for Abaqus to implement Tsai-Wu failure criteria for non-composite structures (I work on long...
25 June 2024 2,955 1 View
I need to set a protocol to culture the bone marrow cell. I appreciate it if anyone help me.
22 June 2024 7,562 2 View
I have been trying to grow Nicotiana benthamiana via tissue culture. I used ethanol and sodium hypochlorite with different concentrations and time duration for seeds sterilization and lastly...
18 June 2024 1,756 3 View