Where can PDGF be found most abundantly outside of blood, marrow and recombinant?

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Studying plasma cell cytokine production in vitro?

I am looking to study if plasma cells in one of the models I am working on secrete various levels of cytokines, but I am unsure which stimulation in vitro should be used to look at this? I have...

31 July 2023 9,230 1 View

DNA extraction - how to separate nucleic acids from GAGs?

I have human cells that I've digested in proteinase K and I'm about to precipitate the glycosaminoglycans in the sample using ethanol, but as I understand it this will also precipitate any nucleic...

05 June 2023 7,000 0 View

My research/dissertation topic is “How can teachers adapt their practice to deliver inclusive PE lesson?

Good day, I am currently in year 2 of my Master's. My research/dissertation topic is “How can teachers adapt their practice to deliver inclusive PE lessons to enhance student engagement?” As...

18 December 2022 4,911 1 View

What sampling method to use for an online survey?

I am aiming to distribute an online survey through social media with the target audience being generation z consumers that purchase in the fashion industry. I wasn't sure what type of sampling...

06 November 2022 1,265 3 View

The Traumatic Stress Institute Belief Scale?

I am attempting to access The Traumatic Stress Institute Belief Scale for my final year project, and I can not seem to able to access it anywhere. Does anyone know where I can find the actual scale?

19 January 2022 4,281 1 View

Is titin expressed in AC16 cells?

Hi there! I need to examine modifications of titin in cardiomyocyte culture. Since I won't collect enough protein if I use primary culture (needs to be adult), I was wondering if anyone has looked...

09 March 2021 4,040 0 View

Do you know someone with knowledge of France in 1700s?

I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...

01 October 2020 9,406 5 View

How to detect gamma H2AX and cleaved caspase 3 signal for WB?

I am trying to detect any differences in DNA damage/apoptosis related proteins by western blot between wild type and disease cells. I ran an initial WB and saw no bands for either protein and am...

18 November 2019 7,377 4 View

How do you measure CO2 in Binder Incubators?

We have two Binder incubators which we'd like to check the CO2 concentration in. On our Thermo incubators we used a Fyrite Gas Analyzer but these incubators don't seem to have a port to measure...

14 November 2019 7,722 1 View

How to calculate error propagation of glacier mass change?

Hi, I am trying to calculate the overall error of my glacier mass change calculations. I have identified the following sources of uncertainty: Volume - Area change of glacier (+/- 3%) -...

17 February 2019 824 2 View

any study on protective factors of care givers of dementia and resilience ?

need to review articles on care giving in dementia and challenges faced

12 August 2024 6,424 1 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

Good day!! All, can anyone recommend the best battery related journals to download research articles please?

I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...

01 August 2024 6,516 2 View

About impact factor?

I have been publishing the Nepalese Journal of Agricultural Sciences (online ISSN 2091-0428; print ISSN 2091-042X) on www.nepjas.com and research gate. I was wondering how I can get an impact...

01 August 2024 1,277 1 View

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (E-ISSN 1901-9726, P-ISSN : 0900-9671) a legitima?

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...

01 August 2024 629 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

30 July 2024 1,412 8 View