Do you know someone with knowledge of France in 1700s?

I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...

01 October 2020 9,095 5 View

How to detect gamma H2AX and cleaved caspase 3 signal for WB?

I am trying to detect any differences in DNA damage/apoptosis related proteins by western blot between wild type and disease cells. I ran an initial WB and saw no bands for either protein and am...

18 November 2019 7,204 4 View

How do you measure CO2 in Binder Incubators?

We have two Binder incubators which we'd like to check the CO2 concentration in. On our Thermo incubators we used a Fyrite Gas Analyzer but these incubators don't seem to have a port to measure...

14 November 2019 7,586 1 View

Any open access meterological data for Spiti Valley Himachal Pradesh?

Hello, I am doing some research for my dissertation and require meterological (ppt and temp) data for Spiti Valley, Himachal Pradesh. Although I have found some data for the Lahul and Spiti...

15 October 2018 7,769 4 View

How to interpret Co-Correspondence analyses (CoCA)?

I'm trying to interpret the results of Co-correspondence analysis (CoCA) with the R package cocorresp. The descriptive paper is rather unclear about how to interpret the results. I've performed...

25 February 2018 2,999 2 View

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?

20 March 2016 3,437 2 View

Can isopropyl alcohol be used to dissolve sucralfate in place of ethanol?

13 March 2016 4,920 2 View

Can someone help me to calculate electrochemical surface area (ECSA) ?

14 December 2015 5,519 7 View

Can doxorubicin affect protein migration on SDS-PAGE?

I have been treating cardiomyocytes with various amounts of doxorubicin (between 250nM-2uM) and running my cell lysates (20ug protein in RIPA with protease /phosphatase/deacetylase inhibitors) on...

24 June 2015 8,712 3 View

How can I make a petroleum ether extract of a plant?

If I have a powdered or ground plant, how can I make a petroleum ether extract of it?

13 April 2015 2,009 15 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View