Does anyone know how long PGE2 will stay stable in PBS? Also, how can I determine whether it will stay stable in a substance such as emu oil?
I am looking to study if plasma cells in one of the models I am working on secrete various levels of cytokines, but I am unsure which stimulation in vitro should be used to look at this? I have...
31 July 2023 9,230 1 View
I have human cells that I've digested in proteinase K and I'm about to precipitate the glycosaminoglycans in the sample using ethanol, but as I understand it this will also precipitate any nucleic...
05 June 2023 7,000 0 View
Good day, I am currently in year 2 of my Master's. My research/dissertation topic is “How can teachers adapt their practice to deliver inclusive PE lessons to enhance student engagement?” As...
18 December 2022 4,911 1 View
I am aiming to distribute an online survey through social media with the target audience being generation z consumers that purchase in the fashion industry. I wasn't sure what type of sampling...
06 November 2022 1,265 3 View
I am attempting to access The Traumatic Stress Institute Belief Scale for my final year project, and I can not seem to able to access it anywhere. Does anyone know where I can find the actual scale?
19 January 2022 4,281 1 View
Hi there! I need to examine modifications of titin in cardiomyocyte culture. Since I won't collect enough protein if I use primary culture (needs to be adult), I was wondering if anyone has looked...
09 March 2021 4,040 0 View
I am in search of those who are familiar with the French & European societies at the end of 1700s to join me in writing a book as my co-authors. The book is on Marie Antoinette. Looking at...
01 October 2020 9,406 5 View
I am trying to detect any differences in DNA damage/apoptosis related proteins by western blot between wild type and disease cells. I ran an initial WB and saw no bands for either protein and am...
18 November 2019 7,377 4 View
We have two Binder incubators which we'd like to check the CO2 concentration in. On our Thermo incubators we used a Fyrite Gas Analyzer but these incubators don't seem to have a port to measure...
14 November 2019 7,722 1 View
Hi, I am trying to calculate the overall error of my glacier mass change calculations. I have identified the following sources of uncertainty: Volume - Area change of glacier (+/- 3%) -...
17 February 2019 824 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey
05 August 2024 5,332 3 View
How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?
01 August 2024 723 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...
22 July 2024 5,492 6 View
Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...
21 July 2024 1,632 0 View