I have a few questions that may seem like beginner questions, but I would really like to know what I must consider when making a solution and keeping efficacy.
I will be trying to make a topical solution of Prostaglandin E2. Here are a few things I want know:
1.) It says Prstaglandin E2 is soluble in water. If I wanted to mix the water/Prostaglandin E2 solution with Emu oil, would that be possible? Or would it loose its efficacy?
2.) If I wanted to make it more gel-like with a thick consistency, how would I do that without loosing efficacy?
3.) Also, how long do you think the solution would stay viable after it had been dissolved in water?
I just want to know everything I have to consider when making a topical solution.
Your whole question is concentrated on two main point:
1. solubility
2. stability
Solublity:--------------------------------------------------------------
Emulsion is of two type oil in water phase (little amount of oil mixed with bulk of water) and water in oil (little amount of water mixed with bulk of oil).
Keep in mind both phase are by nature immisicible with each other but by using energy or surfactant or emulsifying agent they are mixed to each other.
These emulsifying agent / surfactant act in same way as we use detergent to remove greasy substance from our skin/ hand . without surfactant oil not solublise or remove with normal water but when we use detergent they get solublised in water and removed away from our skin toward running water.
so if you want to solublise a water soluble substance to a oil phase then you have to enhance solublity of substance by either using polymers or using surfactant.
Amount of polymer and surfactant vary according to nature of oil phase and nature of product so it can be estimated by experiment only.
stability:-------------------------------------------------------------------
stability of drug in presence of other ingredient is depend on amount and chemical nature and behavior of the excipient present there.
few compund are acid liable and can degrade in presence of acidic media or acid reach oil while some can be degraded in presence of water or some in presence of alcohol or phenol.
Better way to analyse whether any excipient are compatible or not it can be analysed by uv/hplc degradation study.
FTIR is also used to find whther they react with each other and form a new bond (showing different FTIR band).
DSC also used for same purpous as of FTIR.
Using HPLC/UV you can identify whether the amount of drugs were decreasing wrt time if yes then at which rate (slow or fast) so on basis of degration rate you can identify shelf life/ expiry date of your final formulation (maximum time after which atleast 90 % drugs reamins present there)
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