I'm not sure whether this article may help. Hope it will help :

http://usrexp-sandbox.nature.com/nprot/journal/v8/n1/full/nprot.2012.142.html

"Isolating lipid droplets from multiple species". Yunfeng Ding,Shuyan Zhang,Li Yang,Huimin Na,Peng Zhang,Huina Zhang,Yang Wang,Yong Chen,Jinhai Yu,Chaoxing Huo,Shimeng Xu,Martina Garaiova, Yusheng Cong& Pingsheng Liu

Nature Protocols 8, 43–51(2013) doi:10.1038/nprot.2012.142

Try searching for paper by Anthony Cryer but you'll need to go way back to 1980s or even late 70s. I found this one whihc might give you a place to start.

The isolation and characterization of a proposed adipocyte precursor cell type from bovine subcutaneous white adipose tissue.H A HA Plaas and A A Cryer

J Dev Physiol 2(5):275-89 (1980), PMID 7252095

... or even easier this one

Cell and Tissue Research

July 1977, Volume 181, Issue 2, pp 197-203 Isolation of fat cell precursors from adult rat adipose tissue

L. R. Van Robin, Dr. Daniel A. K. Roncari

i suggest you the following protocol

Isolation and Culture of Preadipocytes

from Rodent White Adipose Tissue

Dorothy B. Hausman, Hea Jin Park, and Gary J. Hausman

you might be also interested in this following protocol

Isolation of preadipocytes from rat adipose tissue in vitro.

Katoh J, Ishida Y, Taniguchi H.

Hereby its abstracts :

Fibroblast-like cells, which would be differentiated from mature adipocytes in "ceiling culture", could be isolated in primary culture. These cells precipitated, were collected from the bottom of the flask of the "ceiling culture" and further proliferated to the confluence. They finally converted to morphologically and biochemically differentiated adipocytes. In these investigations we show how to isolate fibroblast-like cells and to identify these cells as "preadipocytes"

Finally you may try this

Preadipocyte Isolation and Culture

Out of freshly excised human subcutaneous adipose tissue of young adults (18-29 years old) who

underwent elective operations, preadipocytes were isolated. This was approved by the Ethical

Committee of the Aachen University of Technology. Using dissecting microscope, vessels and

fibrous tissue were removed, the adipose tissue was minced and digested by collagenase 0.1

U/ml/dispase 0.8 U/ml (Boehringer Mannheim, Mannheim, Germany) at 37°C for 60 min.

Digestion was stopped by adding Dulbecco´s modified Eagle medium (DMEM) containing 15%

foetal calf serum (FCS, Biochrom, Germany) and incubated in erythrocyte lysis buffer (154 mmol/l

NH4

Cl, 10 mmol/lKHCO3, 1 mmol/lEDTA, 10 minutes). The cell suspension was centrifuged

(200xg at 17°C for 10 minutes) and the cells were seeded on tissue culture dishes (63.6 cm2, Greiner,

Germany) with DMEM 15% FCS (added 100 U/mlpenicillin, 100µg/ml streptomycin) with a

seeding density of 3x104 cells/cm². The cells were cultured at 37°C at 10% CO2 and 95% humidity

(Fig. 1). Medium was changed on day 2 and supplemented with EGF (epidermal growth factor,

10ng/ml, Sigma, USA).

Please let me know if you need more information.....wish you Good luck !

We isolated hADSCs from human subcutaneous adipose tissue by adapting the mothod described by Gimble et al 2007 (Gimble, J.M.; Katz, A.J.; Bunnell, B.A. Adipose-derived stem cells for regenerative medicine.

Circ. Res. 2007, 100, 1249–1260.). If it helps, please find more details in our papr: Modulation of Adipogenic Conditions for Prospective Use of

hADSCs in Adipose Tissue Engineering, Int. J. Mol. Sci. 2012, 13, 15881-15900; doi:10.3390/ijms131215881.

Magnetic beads or cell sorting might help purifying the primary cell culture obtained by collagenase digestion.

Good luck !