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Questions related from Bhakta Prasad Gaire
Hello colleague ! I am looking for a good NLRP3 antibody that can be used in human brain section staining. I tried couple of rat/rabbit antibodies that were claimed to be reactive for human as...
27 April 2022 9,087 0 View
Some proteins (abnormal proteins!?) expression pattern is highly different among individual sample so that it is very hard to get the significance difference between the group. For example, the...
28 January 2021 3,864 4 View
I had to perform RNA extraction from the mice skin sample (shaved and hair removed tissue sample of about 1*1 cm square). For that I pulverized the tissue in liquid nitrogen pre-chilled mortar and...
21 December 2018 941 3 View
Dear colleague Many researches stated the semi-therapeutic effect of regimen in mice model of Imiquimod induced psoriasis like skin disease. That is IMQ and the drug are applied for almost...
11 October 2017 9,548 1 View
I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...
07 September 2016 3,449 1 View
Hello everyone ! I got some strange result following ischemic experiment in mice. After 3 days of MCAO/reperfusion microglia are believed to be activated and morphologically transformed to the...
02 June 2016 5,299 3 View
I have one natural compound that has ROS production inhibitory effect in vitro at 1 micro-gram/ml concentration. I wish to use this compound in mice model of focal cerebral ischemia How can I...
30 May 2016 8,588 6 View
I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I...
29 March 2016 7,663 6 View
In many cases, neurological score and infarct volumes of MCAo/reperfusion (M/R) induced brain damage correlates. I mean if infarct volume is improved so does the neurological functions. However,...
29 February 2016 3,199 2 View
I am willing to do TUNEL staining in transient focal cerebral ischemia challenged mouse brain section to observe the extent of apoptosis. I have got lots of different methodologies mentioned in...
15 December 2015 5,922 4 View
I am currently working in Sepsis brain model in mice. I have to use the mouse brain for immunohistochemistry and RNA synthesis purpose. As we know for immunohistochemistry purpose we perfuse mouse...
17 July 2015 2,474 6 View
I hope there might be the particular software through which we can draw the cell membrane, lymphocytes cells, glial cells and sometimes brain slices. Do you know any of these software so that we...
17 June 2015 4,813 6 View
Does anybody have the pharmacokinetic data that reflects the drug absorption time in mice after oral administration ? Such as 2 hr or 3 hr. In my case the drug that is impermeable to the BBB was...
25 March 2015 1,071 4 View
AUY954 is selective S1P1 agonist and analogue of well known non selective S1P receptor agonist FTY720. FTY do internalize the S1P1 upon binding to the receptor and prevents its recycling to the...
06 February 2015 1,999 2 View
A man was diagnosed as UC patients after colonoscopy about a month and half ago. After that he is taking the medicine sulfasalazine and steroid to overcome the symptoms of UC. The dose of steroid...
27 January 2015 4,234 8 View
Sphingosine-1-phosphate1 (S1P1) regulates various molecular and cellular events in different body parts. Its expression pattern and functions are also varied dependent upon the cells types where...
21 January 2015 3,140 4 View
I wish to know the detecting of proliferated microglia after 3 days of stroke induction. Some of paper suggested the Brudu immunohistochemistry analysis for this purpose but they use transgenic...
06 November 2014 9,659 2 View
I am doing the research on animal stroke models. Generally we use the MCAo induced focal cerebral ischemic models in mice. As it is obvious that mice undergone with right MCA occlusion circles...
03 September 2014 6,321 1 View
I am working in cerebral ischemic models. I generally used in vivo models in mice or rat for the ischemia induction. I am also interested in in vitro ischemic models such as OGD-R. Would be very...
01 July 2014 5,977 3 View
Iba 1 is used for the immunohistochemical analysis of activated microglial cells. I am doing stroke animal research and frequently encounter the staining with the Iba 1 antibody. My problem is Iba...
23 May 2014 9,058 3 View
I have to perform the QPCR experiment in order to determine the fold increase or decrease of several cytokine levels. In an experiment I found that one cytokine's mRNA level increased about 60...
17 April 2014 2,682 2 View
I administered 1 microlitre/min of lentivirus in the brain ventricle in order to knockdown a specific gene. However, generally there is no knockdown or little knockdown success. In this case, how...
25 March 2014 3,547 2 View
In MCAO experiment, the infarct volume is calculated by using TTC staining. The white colored portion in ipsilateral hemisphere is considered as infarct portion. However in my experiment, in a...
14 January 2014 1,117 3 View
I am performing the neurological research and wish to know how to perform the sectioning of the hypothalamus. So that I can stain and perform further a study.
06 December 2013 178 3 View
I am working with the ICR mice for BCCAo. After 15 to 20 min occlusion, I found that there was no neuronal cell deaths in hippocampal CA1 region. Could anyone explain to me if this is the impact...
25 October 2013 3,416 4 View
I am working in global cerebral ischemia and to evaluate the neuronal cell death we have to section the animal brain and see the hippocampal neuronal cell density. After Nissl staining I found...
18 October 2013 9,724 1 View
Do you have any articles regarding the protective effect of Terminalia chebula on the glutamate induced toxicity to the neurons? Both in vitro and in vivo paper will be appreciated. Either the...
23 April 2013 725 2 View
I am planning to prepare female MCAo stroke model without estrogen. The removing the ovary of the female SD rat is the popular and most widely used method for that. I have undergone through the...
22 March 2013 1,345 1 View
I am going to perform a study related to the neuroprotective effect of certain herbal compounds and formula in ovariectomized female SD rats through the middle cerebral artery occlusion (MCAo)...
15 February 2013 7,999 0 View
