Bhakta Prasad Gaire

9 Questions 21 Answers 0 Followers

Questions related from Bhakta Prasad Gaire

A good NLRP3 antibody that works for human brain immunostaining?

Hello colleague ! I am looking for a good NLRP3 antibody that can be used in human brain section staining. I tried couple of rat/rabbit antibodies that were claimed to be reactive for human as...

27 April 2022 9,034 0 View

How to get rid of significance issues in Western blot quantification of irregularly expressed proteins between samples?

Some proteins (abnormal proteins!?) expression pattern is highly different among individual sample so that it is very hard to get the significance difference between the group. For example, the...

28 January 2021 3,760 4 View

Is there anything wrong in RNA extraction from mouse skin sample ?

I had to perform RNA extraction from the mice skin sample (shaved and hair removed tissue sample of about 1*1 cm square). For that I pulverized the tissue in liquid nitrogen pre-chilled mortar and...

21 December 2018 856 3 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

07 September 2016 3,365 1 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

In many cases, neurological score and infarct volumes of MCAo/reperfusion (M/R) induced brain damage correlates. I mean if infarct volume is improved so does the neurological functions. However,...

29 February 2016 3,129 2 View

Why does the mouse with right MCA occlusion circle to the left?

I am doing the research on animal stroke models. Generally we use the MCAo induced focal cerebral ischemic models in mice. As it is obvious that mice undergone with right MCA occlusion circles...

03 September 2014 6,263 1 View

Does Iba 1 stain Sham operated brain slices?

Iba 1 is used for the immunohistochemical analysis of activated microglial cells. I am doing stroke animal research and frequently encounter the staining with the Iba 1 antibody. My problem is Iba...

23 May 2014 9,011 3 View

Does Terminalia chebula have the protective effect in glutamate toxicity?

Do you have any articles regarding the protective effect of Terminalia chebula on the glutamate induced toxicity to the neurons? Both in vitro and in vivo paper will be appreciated. Either the...

23 April 2013 637 2 View

What is the best age for MCAo ovariectomy rat?

I am planning to prepare female MCAo stroke model without estrogen. The removing the ovary of the female SD rat is the popular and most widely used method for that. I have undergone through the...

22 March 2013 1,297 1 View