How to get rid of significance issues in Western blot quantification of irregularly expressed proteins between samples?

Some proteins (abnormal proteins!?) expression pattern is highly different among individual sample so that it is very hard to get the significance difference between the group. For example, the...

27 January 2021 3,629 4 View

How much sample size is necessary for the cross sectional study done in school/college level students about their smoking and drinking behaviors?

26 September 2020 8,101 7 View

Can anyone help to to find following values for Concrete 02 in OpenSees (Without experiment)?

$fpcu = concrete crushing strength * $epsU = concrete strain at crushing strength*

16 May 2020 7,427 6 View

Is there anything wrong in RNA extraction from mouse skin sample ?

I had to perform RNA extraction from the mice skin sample (shaved and hair removed tissue sample of about 1*1 cm square). For that I pulverized the tissue in liquid nitrogen pre-chilled mortar and...

20 December 2018 774 3 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

06 September 2016 3,267 1 View

Can anybody suggest a way to identify a particular protein from yeast ?

I want to identify a particular protein from yeast. I tried to do MSMS. But it's never giving any hit with any protein from Saccharomyces cerevisiae. For your information I have only the SDS PAGE...

03 May 2016 1,500 5 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

28 February 2016 3,044 2 View

Does Terminalia chebula have the protective effect in glutamate toxicity?

Do you have any articles regarding the protective effect of Terminalia chebula on the glutamate induced toxicity to the neurons? Both in vitro and in vivo paper will be appreciated. Either the...

22 April 2013 564 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View