How to get rid of significance issues in Western blot quantification of irregularly expressed proteins between samples?

Some proteins (abnormal proteins!?) expression pattern is highly different among individual sample so that it is very hard to get the significance difference between the group. For example, the...

27 January 2021 3,629 4 View

How much sample size is necessary for the cross sectional study done in school/college level students about their smoking and drinking behaviors?

26 September 2020 8,101 7 View

Can anyone help to to find following values for Concrete 02 in OpenSees (Without experiment)?

$fpcu = concrete crushing strength * $epsU = concrete strain at crushing strength*

16 May 2020 7,427 6 View

Is there anything wrong in RNA extraction from mouse skin sample ?

I had to perform RNA extraction from the mice skin sample (shaved and hair removed tissue sample of about 1*1 cm square). For that I pulverized the tissue in liquid nitrogen pre-chilled mortar and...

20 December 2018 774 3 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

06 September 2016 3,267 1 View

Can anybody suggest a way to identify a particular protein from yeast ?

I want to identify a particular protein from yeast. I tried to do MSMS. But it's never giving any hit with any protein from Saccharomyces cerevisiae. For your information I have only the SDS PAGE...

03 May 2016 1,500 5 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

28 February 2016 3,044 2 View

Does Terminalia chebula have the protective effect in glutamate toxicity?

Do you have any articles regarding the protective effect of Terminalia chebula on the glutamate induced toxicity to the neurons? Both in vitro and in vivo paper will be appreciated. Either the...

22 April 2013 564 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

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01 March 2021 3,905 5 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View