I am working with the ICR mice for BCCAo. After 15 to 20 min occlusion, I found that there was no neuronal cell deaths in hippocampal CA1 region. Could anyone explain to me if this is the impact of posterior communicating artery? And do all ICR mice have both PcomA? How can we evaluate whether a mouse strain has PcomA or not?
Hi Bhakta,
all mice, no matter if inbred strain or not, have a well developed circulus arteriosus in the brain with very sufficient anterior and porterior communicantes and further onnections between both hemispheres.
Therefore most researchers use Gerbils as model for stroke/ cerebral ischemia. Gerbils lack the communicantes.
Hope this Helps.
Dear Bhakta,
I agree with Frauke but I want to add that there is evidence that different mouse strains show vascular anatomy related differences in suscebtibility to ischemia. For your work you should examine the middle cerebral artery (MCA) trunk and branch as well as the presence/absence of the posterior communicating arteries (PComAs) or other anastomosing vessels (you can perfuse mice with black ink). The development of left and right PComAs should be scored individually (e.g. absent: 0;
present but hypoplastic or other anastomosing vessels: 1; present and
well formed: 2) and should be calculated for each animal by averaging both, left and right scores. Maybe these two publications are helpful for you...
Katrin
- Barone, F.C., Knudsen, D.J., Nelson, A.H., Feuerstein, G.Z., Willette, R.N., 1993. Mouse strain differences in susceptibility to cerebral ischemia are related to cerebral
vascular anatomy. J. Cereb. Blood Flow Metab. 13, 683–892.
- Kleinschnitz, C., Schwab, N., Kraft, P., Hagedorn, I., Dreykluft, A., Schwarz, T., Austinat, M., Nieswandt, B., Wiendl, H., Stoll, G., 2010. Early detrimental T-cell effects in experimental cerebral ischemia are neither related to adaptive immunity nor
thrombus formation. Blood 115, 3835–3842.
Answers for your questions were in our following publication:
C57BL/6 strain is most ------. Brain Res 752: 209-218, 1997
Thank you all for your valuable support. So C57BALB/6 is the best mouse strain in order to use in BCCAo mouse animal model?
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