I am working in global cerebral ischemia and to evaluate the neuronal cell death we have to section the animal brain and see the hippocampal neuronal cell density. After Nissl staining I found that there are water droplet like structure within the cell line of hippocampal CA1,2,3 region. I am using PBS as pre perfusion solution and pfa as perfusion fixed solution. After blocking by sucrose I preserve the tissue mold in -80 Celsius and performed the sectioning using microtome. Could anyone experiences the similar problem and resolve this problem?
@Bhakta Prasad. Though I am not very much familiar with this kind of problem, I would like to ask back that have you ruled out the possibilities of extraneous material or air bubbles? Moreover, did you try the other procedure without using sucrose?
- Badges
- Science topic