I am willing to do TUNEL staining in transient focal cerebral ischemia challenged mouse brain section to observe the extent of apoptosis. I have got lots of different methodologies mentioned in literatures or online as well. However they lack detail experimental protocol. Could you please forward me the suitable protocol for TUNEL staining in detail ? it would be better if you send me the one that you are applying currently in your lab. I will use this technique for OCT preserved frozen brain section which are pefused with 4% praformaldehyde.
I've been using the In Situ Cell Death Detection Kit from Roche (attached below) and it works very well, and it's simple. You have different procedures based on the type of your samples, and that's an advantage. I think if you want to use your own reagents and solutions instead of buying the kit, you may try following the steps described on the kit protocol.
Hope this can be helpful.
Ana
Hi~Bhakta. How are you?
I'm sorry that I don't know the TUNEL staining protocol in detail. In fact, I have not been to do this experiment.
Good luck in your study.
I am very fine Jin Gyu bro. thanks for your message. hope you are doing well there as always !! Good luck to you too!
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