Does anybody have the pharmacokinetic data that reflects the drug absorption time in mice after oral administration ? Such as 2 hr or 3 hr. In my case the drug that is impermeable to the BBB was orally pre-treated 1 hr before MCAo surgery but the drug still showed the neuroprotective effect. So at this case how can I discuss the result? If the drug absorption time is about 2 hr then we can say BBB rupture and drug is penetrating the brain, or does the drug has indirect neroprotective effect?
The pharmacokinetics (PK) of drug results from the interplay of the drug properties and the context (species, age, diet, etc), so there is no general answer to your question. If plasma PK is known, you know what concentration-time profile the brain is exposed to. Note that the unbound drug pharmacokinetics are important, as driving BBB transport. Then, BBB transport has a rate (how fast will the drug pass the BBB) and brain distribution will have an extent. The pharmacokinetics of the unbound drug at the target site is what should be linked to the effect.... so there is quiet a bit to be know before drawing firm conclusions...
Generally after oral administration drug takes approx. 1 hr, but it varies for different drug, time may increase or decrease depending upon drug characteristics and formulation characterisation;
during mcao due to injury there is rupturing of bbb, due to that the drug which generally not crosses bbb can cross the bbb and shows neuroprotective effects, alongwith this there can be possibility of some other pathways.............
The drug absorption rate in mice would be dependent on the medication being administered.
integrity of the blood brain barrier of disrupted after an MCAO or in original stroke through activation of various serine proteases. You may use inhibitors of these proteases to eveauate weather your molecule gor into brain by its own or you may try its pharmakokinetics in healty animals first...
read this article too...