In MCAO experiment, the infarct volume is calculated by using TTC staining. The white colored portion in ipsilateral hemisphere is considered as infarct portion. However in my experiment, in a vehicle treated group, there is intense while color, while dim white color is obtained in drug treated group. You can see in the attached photograph as well. In this case can I say the drug is protective or not? Including the concentration of TTC, time of TTC staining, all other conditions were the same as sham, vehicle and drug treated group. The neurological scores were improved in drug treated group than in the vehicle.
Yes hemorrhage play the similar role and seems the dim infarct factor. But in many cases we got the similar results without any visible spot of hemorrhage. At that case how can we interpret the results?
Benedek A, Moricz K, Juranyi Z et al. Use of TTC
staining for the evaluation of tissue injury in the early
phases of reperfusion after focal cerebral ischemia in
rats. Brain Res 2006; 1116: 159–165.
Formation of formazan from 2,3,5-triphenyltetrazolium
chloride (TTC) can depend on both the staining method
(in vitro and in vivo) and the metabolic burdens of the
brain tissue (intensity of and recovery from ischemia).
These caused uncertainties in the volume of ischemia
which induced brain injury measured by TTC staining. To
measure accurate size of infarction, we have applied the
histopathological measurement under the microscopy
instead of TTC.
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