What is the best age for MCAo ovariectomy rat?

22 March 2013 1 1K Report

I am planning to prepare female MCAo stroke model without estrogen. The removing the ovary of the female SD rat is the popular and most widely used method for that. I have undergone through the several research articles which have been using the same models.

Each and every articles provide the weight of the rats but many of them did not mention the age. And there was large variations in the age of rats used in the experiments from one article to other, though the weight is almost same. So I am very much confused that what aged animal should be used for the appropriate outcomes while preparing the animal stroke models.

Morteza Jourkesh

In my opinion,the best

age for female rats OVX is 12 weeks old.

Badges
Science topic

More Bhakta Prasad Gaire's questions See All

How to get rid of significance issues in Western blot quantification of irregularly expressed proteins between samples?

Some proteins (abnormal proteins!?) expression pattern is highly different among individual sample so that it is very hard to get the significance difference between the group. For example, the...

27 January 2021 3,629 4 View

How much sample size is necessary for the cross sectional study done in school/college level students about their smoking and drinking behaviors?

26 September 2020 8,101 7 View

Can anyone help to to find following values for Concrete 02 in OpenSees (Without experiment)?

$fpcu = concrete crushing strength * $epsU = concrete strain at crushing strength*

16 May 2020 7,427 6 View

Is there anything wrong in RNA extraction from mouse skin sample ?

I had to perform RNA extraction from the mice skin sample (shaved and hair removed tissue sample of about 1*1 cm square). For that I pulverized the tissue in liquid nitrogen pre-chilled mortar and...

20 December 2018 774 3 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

06 September 2016 3,267 1 View

Can anybody suggest a way to identify a particular protein from yeast ?

I want to identify a particular protein from yeast. I tried to do MSMS. But it's never giving any hit with any protein from Saccharomyces cerevisiae. For your information I have only the SDS PAGE...

03 May 2016 1,500 5 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

28 February 2016 3,044 2 View

Does Terminalia chebula have the protective effect in glutamate toxicity?

Do you have any articles regarding the protective effect of Terminalia chebula on the glutamate induced toxicity to the neurons? Both in vitro and in vivo paper will be appreciated. Either the...

22 April 2013 564 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

CT image preprocessing in python?

I have a 3D CT image of a car engine in raw form with no header information. When loaded into numpy array as 16 bit unsigned integer, I noticed that the values range between 0 to 52000. Is this...

15 February 2021 4,736 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Hyperspectral Image : open, visualize, process in Python?

I am looking for a tutorial or course to learn how to open, visualize , process hyperspectral images in .hdr , .raw format using python. Any recommendation for a tutorial is appreciated ? Thank...

08 February 2021 7,896 3 View