I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I will be happy if you let me know the length of monofilament from the carotid bifurcation as well. I am making mono-filament by myself and will be happy if you provide me the source of commercial use of mono-filament.
For 7 week age mice you can use a 5-0 mono filament suture with tip diameter of 0.21-0.22 mm. From carotid bifurcation you have introduce a distance of 8-10 mm. You can get filament from Harvard Apparatus.
I agree with Mr. Dibbanti. for such a small mice, 5-0 from coviden, called Dermalon monofilament nylon. you can buy this from fisher or harvard apparatus.
hope this helps!
Sandra
regarding the filament diameter you should consider the animal body weight
check this out!
https://www.doccol.com/information.php?info_id=15
We use silicon rubber-coated monofilaments from Doccol for our 8wk C57s: size 6-0, diameter 0.09-0.11 mm; diameter with coating 0.22 +/- 0.02 mm, and coating length 1-2 mm (Catalog No.: 602212PK10). Doccol also has great reusable filaments that have produced consistent tMCAO with minimal post-stroke hemorrhage, in our experience. As Dibbanti noted, insertion length is typically 8-10mm but should always be verified using an LDF to measure cerebral blood flow (CBF), especially in mice. We mark out filaments with a paint pen at 8mm, and monitor CBF throughout the surgery. Another important aspect if buying commercially is tip length. Keep it relatively short (2mm), or you will unintentionally block other arterial flow.
www.doccol.com
Dear colleagues
Thank you very much for your nice comments. I am hoping other more experiences to set up my experiment.
Thank you all
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