I wish to know the detecting of proliferated microglia after 3 days of stroke induction. Some of paper suggested the Brudu immunohistochemistry analysis for this purpose but they use transgenic mice. I wish to know how to detect proliferative microglia in normal ICR mice. If you have some experience please suggest me.
Why don't you start by simply counting the number of Iba-1 positive cells and comparing it to a control animal?
Double immunofluorescence on slices with IBA1 or F480 (the best marker for mouse microglia, both resting and activated) and Ki67. If your microglia is activated, as is expected after stoke, you can also use Cd11b antibodies (rat mAb) and anti-Ki67 (poly Rb). This is fine for your purpose. Slices can be fixed in PFA 4% before staining.
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