I administered 1 microlitre/min of lentivirus in the brain ventricle in order to knockdown a specific gene. However, generally there is no knockdown or little knockdown success. In this case, how can I increase the success rate of ICV-induced knockdown of a specific receptor or gene?
1 microlitre/min is high speed and might not allowing the virus to diffuse into the brain. Here are my suggestions, you can try slow down the speed (try 0.1 to 0.3 microlitre/min), use the 27 guage needle, and allow the needle in place after injecting the whole amount in place for at least 5-10 minutes (this will give the virus enough time to diffuse). Also make sure that you do not have the air bubble in your syringe. You can minimize that by aspirating 0.5 micrlitre ddw and have a 0.3 microlitre air gap in syringe and then take the virus (I guess you must be injecting about 2 microlitre, you can also try more concentrated virus and reduce the volume to 1 microlitre). See this article for reference
good luck
http://www.pnas.org/content/106/38/16505.full
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