In some questions asked by others, I see people categorizing circulating miRNA as only miRNA from serum and plasma, shouldn't miRNA from whole blood also be considered circulating miRNA?
EVEN MIrnaS BOUND TO HDL in ehole blood are considered a part of circulting miRNAS i suppose
Circulating cells in the blood have intracellular microRNAs. These are not considered circulating microRNAs.
I dont think so.. As Maged Harraz also saying, blood has its own cells and they also carry their intracellular miRNA; therefore miRNAS recovered only from serum/plasma is considered as circulating miRNA, not from whole blood.
HI all...
There is differential miRNA profile in normal and diseased (abnormal) conditions ...so the point is the profile of the miRNAs of normal person should be considered within the domain... but the miRNAs that increased in the diseased condition should not be condisered within this repertoire of whole blood miRNAs..
cheers...
Hello Marc,
I agree with Isha and to some extent with Shokat as well.
It is interesting that you asked about circulating miRNas and the consideration of serum and/or plasma.
Honestly speaking, based on the diseased condition, it is best to work on miRNA extracted from serum and/or plasma. Whole blood may have external/alien miRNA as well. We recently published a hypothesis based on similar lines:
https://www.researchgate.net/publication/301578307_Are_We_Eating_Our_Way_to_Prostate_Cancer-A_Hypothesis_Based_on_the_Evolution_Bioaccumulation_and_Interspecific_Transfer_of_miR-150
However, to justify, that any particular miRNA has its origin in a particular diseased tissue, it is very difficult, as tissue-origin and "circulating" miRNA are two vey different beasts.
Hope this helps.
Warm regards,
Venk
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