Showkat Ahmad

4 Questions 6 Answers 0 Followers

Questions related from Showkat Ahmad

Which statistical significance test to use for siRNA based knockdown assay? Is ANOVA-(one way) good enough for this experiment?

We performed the siRNA based knockdown assay with 10 different siRNAs and each siRNA with three different concentrations (0.1, 0.5, 1 nM) and repeated three times in triplicates (3-wells) each...

10 October 2017 3,493 4 View

PAGE for siRNA at different time points for serum stability with etbr.

I need to perform PAGE for siRNA at different time points for serum stability with etbr. Please share your protocol etc especially for using etbr post running the siRNAs. Thanks in advance.

08 August 2016 8,131 0 View

Dual luciferase assay luminescence experiment (promega E2920)--guidance needed?

Greetings...I am performing experiment of knockdown using siRNA with dual glo luciferase assay system E2920 (promega) and psi-check-TM2 plasmid1) what instrument should I use for two readings and...

05 May 2016 5,735 6 View

How can we express the viral protein that is not complete ORF (i.e. with out start or stop codon) but a part of one complete orf ?

Showkat Ahmad How can we express the viral protein that is not complete ORF (i.e. with out start or stop codon) but a part of one complete gene and becomes functional protein after Post...

12 December 2015 6,186 4 View