Marc Paul O'Sullivan

11 Questions 13 Answers 0 Followers

Questions related from Marc Paul O'Sullivan

What additional information do I need for a gene expression a priori sample size calculation?

We want to calculate the sample size for a future study: We have two groups called cases and controls We want to set alpha value at 0.05 We want to set power at 0.8 We want a fold change of...

07 July 2019 1,671 5 View

Why doesn't cell-free DNA immediately degrade in the circulation?

We know that extracellular vesicles and protein bound RNA is stable in the circulation. Can anyone recommend why cell-free DNA doesn't degrade in the circulation?

03 March 2019 6,906 4 View

Can you perform a second log transformation on 2^-DDCT to further reduce the skew?

Can you perform a log transformation, i.e. Log2 on your 2^-DDCT data to reduce the skew, thereby performing parametric tests as these may be more robust and reliable than non-parametric tests.

03 March 2019 7,231 3 View

Citations on log transformation of DDCT-PCR results?

I was recently told at a PCR based workshop that DDCT-PCR data should always be log transformed to the ^2 or ^10 as it allows a normal distribution for your results and allows you to use more...

01 January 2019 5,754 3 View

How to best use miRNA target/pathway prediction software and resources?

I'm sure many people know there are a large number of prediction softwares' available online, with most including high throughput and low throughput validation. Most of these have a filter list,...

06 June 2018 5,967 0 View

MiRNA Upregulated in NGS and Downregulated in RT-qPCR?

We've recently ran an NGS, had it analysed and tried to validate using RT-qPCR? Two of the miRs were significantly changed in both NGS and RT-qPCR but in different directions, i.e. one was...

10 October 2017 1,360 9 View

Regarding how much cDNA is made in a cdna reaction?

If you start off with 20ul of 20ng/ul of total RNA, and you need 4ul of this for a 20ul cDNA reaction (4ul template RNA + 16ul of enzyme and buffer mix). My understanding is I've input 4ul of...

03 March 2017 9,557 3 View

What yields better results microarray or next generation sequencing?

Specifically for miRNA, but also interested in RNA in general

11 November 2016 9,885 7 View

Good reviews on comparisons between serum and plasma?

I'm quite eager to find papers that compare the make up between serum and plasma, that goes beyond the fact that plasma is treated with an anticoagulant, unlike serum which is allowed time to...

11 November 2016 4,774 0 View

What are the main pathways involved in oligodendrocyte development?

Are there any seminal papers on pathways in oligodendrocyte development?

05 May 2016 9,254 2 View

Would miRNAs in whole blood still be considered circulating miRNA, or would this be primarily in serum and plasma?

In some questions asked by others, I see people categorizing circulating miRNA as only miRNA from serum and plasma, shouldn't miRNA from whole blood also be considered circulating miRNA?

05 May 2016 8,686 5 View