What are the main pathways involved in oligodendrocyte development?

More Marc Paul O'Sullivan's questions See All

What additional information do I need for a gene expression a priori sample size calculation?

We want to calculate the sample size for a future study: We have two groups called cases and controls We want to set alpha value at 0.05 We want to set power at 0.8 We want a fold change of...

06 July 2019 1,698 5 View

Why doesn't cell-free DNA immediately degrade in the circulation?

We know that extracellular vesicles and protein bound RNA is stable in the circulation. Can anyone recommend why cell-free DNA doesn't degrade in the circulation?

02 March 2019 6,939 4 View

Can you perform a second log transformation on 2^-DDCT to further reduce the skew?

Can you perform a log transformation, i.e. Log2 on your 2^-DDCT data to reduce the skew, thereby performing parametric tests as these may be more robust and reliable than non-parametric tests.

02 March 2019 7,260 3 View

Citations on log transformation of DDCT-PCR results?

I was recently told at a PCR based workshop that DDCT-PCR data should always be log transformed to the ^2 or ^10 as it allows a normal distribution for your results and allows you to use more...

31 December 2018 5,780 3 View

How to best use miRNA target/pathway prediction software and resources?

I'm sure many people know there are a large number of prediction softwares' available online, with most including high throughput and low throughput validation. Most of these have a filter list,...

05 June 2018 5,999 0 View

MiRNA Upregulated in NGS and Downregulated in RT-qPCR?

We've recently ran an NGS, had it analysed and tried to validate using RT-qPCR? Two of the miRs were significantly changed in both NGS and RT-qPCR but in different directions, i.e. one was...

09 October 2017 1,386 9 View

Regarding how much cDNA is made in a cdna reaction?

If you start off with 20ul of 20ng/ul of total RNA, and you need 4ul of this for a 20ul cDNA reaction (4ul template RNA + 16ul of enzyme and buffer mix). My understanding is I've input 4ul of...

02 March 2017 9,580 3 View

What yields better results microarray or next generation sequencing?

Specifically for miRNA, but also interested in RNA in general

10 November 2016 9,913 7 View

Good reviews on comparisons between serum and plasma?

I'm quite eager to find papers that compare the make up between serum and plasma, that goes beyond the fact that plasma is treated with an anticoagulant, unlike serum which is allowed time to...

10 November 2016 4,803 0 View

Would miRNAs in whole blood still be considered circulating miRNA, or would this be primarily in serum and plasma?

In some questions asked by others, I see people categorizing circulating miRNA as only miRNA from serum and plasma, shouldn't miRNA from whole blood also be considered circulating miRNA?

04 May 2016 8,718 5 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Any tips on how to increase the chances of getting a response?

I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....

31 July 2024 7,771 5 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View