What additional information do I need for a gene expression a priori sample size calculation?

We want to calculate the sample size for a future study: We have two groups called cases and controls We want to set alpha value at 0.05 We want to set power at 0.8 We want a fold change of...

06 July 2019 1,600 5 View

Why doesn't cell-free DNA immediately degrade in the circulation?

We know that extracellular vesicles and protein bound RNA is stable in the circulation. Can anyone recommend why cell-free DNA doesn't degrade in the circulation?

02 March 2019 6,857 4 View

Can you perform a second log transformation on 2^-DDCT to further reduce the skew?

Can you perform a log transformation, i.e. Log2 on your 2^-DDCT data to reduce the skew, thereby performing parametric tests as these may be more robust and reliable than non-parametric tests.

02 March 2019 7,136 3 View

Citations on log transformation of DDCT-PCR results?

I was recently told at a PCR based workshop that DDCT-PCR data should always be log transformed to the ^2 or ^10 as it allows a normal distribution for your results and allows you to use more...

31 December 2018 5,667 3 View

How to best use miRNA target/pathway prediction software and resources?

I'm sure many people know there are a large number of prediction softwares' available online, with most including high throughput and low throughput validation. Most of these have a filter list,...

05 June 2018 5,907 0 View

MiRNA Upregulated in NGS and Downregulated in RT-qPCR?

We've recently ran an NGS, had it analysed and tried to validate using RT-qPCR? Two of the miRs were significantly changed in both NGS and RT-qPCR but in different directions, i.e. one was...

09 October 2017 1,283 9 View

Regarding how much cDNA is made in a cdna reaction?

If you start off with 20ul of 20ng/ul of total RNA, and you need 4ul of this for a 20ul cDNA reaction (4ul template RNA + 16ul of enzyme and buffer mix). My understanding is I've input 4ul of...

02 March 2017 9,491 3 View

What yields better results microarray or next generation sequencing?

Specifically for miRNA, but also interested in RNA in general

10 November 2016 9,831 7 View

Good reviews on comparisons between serum and plasma?

I'm quite eager to find papers that compare the make up between serum and plasma, that goes beyond the fact that plasma is treated with an anticoagulant, unlike serum which is allowed time to...

10 November 2016 4,643 0 View

Would miRNAs in whole blood still be considered circulating miRNA, or would this be primarily in serum and plasma?

In some questions asked by others, I see people categorizing circulating miRNA as only miRNA from serum and plasma, shouldn't miRNA from whole blood also be considered circulating miRNA?

04 May 2016 8,601 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

No title

01 March 2021 3,905 5 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View