Paria Rahnama

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Questions related from Paria Rahnama

Without using NMR technique how can we check exopolysaccharides are completely hydrolyzed?

I used a 2 M trifluoroacetic acid solution and hydrolyzed the EPS at 100°C for 2 h. How can I distinguish this method hydrolyzed the samples completely? I don't have access to NMR

20 May 2022 4,734 4 View

How we can distinguish an exopolysaccharide have how many monosaccharide ?

I used HPLC technique to determine monosaccharide composition of produced exopolysaccharide , I have molar ratios of 0.12 : 0.1: 0.03: 02: 0.06 of Rhamnose, Xylose, Arabinose, Manose+fructose and...

18 March 2020 2,593 3 View

How much H2SO4 is used for hydolysis of exopolysaccharide to characterize monosaccharide composition?

To characterize monosaccharide composition of exopolysaccharide by HPLC method I need to hydrolyze EPS with 0.5 M H2SO4 at 95°C for 12 h followed by neutralization with 4 M NaOH. How much sample...

06 December 2019 5,044 2 View

How much (chloroform: n-butanol = 4:1) can be used for deproteinization of polysaccharide solution?

I am using the Sevag method to the purification of the EPS to remove protein but I don't know how much sevag agent (chloroform: n-butanol = 4:1) should I use?

29 October 2019 3,611 1 View

Is there a certain formula to change time and speed of centrifuge?

For example I am using this protocol for separation in centrifuge: 10000g for 10 min . I want to decrease the time of centrifuge to 5 min, how can I increase the speed of centrifuge? Is the a...

16 August 2019 9,577 3 View

How can I modify boiling method for DNA extraction of gram positive bacteria like lactic acid bacteria?

I used boiling method to extract DNA from lactic acid bacteria. as follow procedure: Fifty microliter of dH2O is added to the pellet. After vortexing, the sample is boiled at 98°C for 10 minutes...

06 June 2019 1,976 4 View

Can I use sodium citrate instead of tri-ammonium citrate (in original formlation) to make a formulated MRS broth?

in other words, doesn't sodium citrate inhibit growth of Lactic Acid Bacteria?

21 January 2019 6,508 1 View

Which method is better?

Which culture method for screening of exopolysaccharide-producing lactic acid bacteria on MRS agar is better? Streak method or spot method? … Read more

28 November 2018 1,256 4 View

Is size exclusion chromatography a proper method for purification of bacterial exopolysaccharide?

I have EPS from lactic acid bacteria and I want to remove carbohydrate residues from medium. I should say I will do these steps befor using size exclusion chromatography: centrifugation TCA...

26 May 2018 7,070 4 View