How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

More Paria Boveiri Dehsheikh's questions See All

Why MiFish Primers amplify when they're alone but not when they have an overhang sequence (Illumina adapter + tag)?

Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...

11 January 2024 5,109 2 View

How to extract total RNA from chondrogenic micromass?

Hi, I am trying to extract total RNA from chondrogenic micromass after 14days of chondrogenesis. I have used Qiagen RNAesay plus mini kit. It was extremely difficult to dissolve the micromass in...

29 November 2023 2,226 2 View

What are available methods for modeling mutual negotiation between players in a coalition game?

In the literature, mutual negotiation hasn't been considered in a cooperative game.

04 June 2023 1,217 0 View

Kindly, suggest me some available methods for modeling distributed decision-making of participants?

As we know, asynchronous energy pricing such as double auction and negotiation allows the execution of several bilateral contracts between prosumers and consumers. In the case of a double auction...

04 June 2023 6,279 0 View

Measurements of sarcomere alignment in thick tissues?

Hello, cardiac tissue engineers! I am a cardiac tissue graduate student, I have fabricated a 50-um cardiac tissue with cardiomyocytes, and want to characterize it, I fixed and stained my tissue...

23 February 2023 3,693 0 View

In SNRS cases What kind of biopsy results lead the physician to Genetic testing directly?

There are currently no guidelines for the use of genetic testing for SRNS in clinical practice. In SNRS cases What kind of biopsy results lead the physician to Genetic testing directly?

05 July 2022 7,976 0 View

Without using NMR technique how can we check exopolysaccharides are completely hydrolyzed?

I used a 2 M trifluoroacetic acid solution and hydrolyzed the EPS at 100°C for 2 h. How can I distinguish this method hydrolyzed the samples completely? I don't have access to NMR

19 May 2022 4,704 4 View

How can i parametrize new compounds to dftb+ library?

Actually, im just new to dftb+, i study many articles and also the manuall for dftb+ of Material Studio , but still so confused about this matter, i wanna to study the adsorption of water on Au...

28 December 2021 5,228 0 View

How we can distinguish an exopolysaccharide have how many monosaccharide ?

I used HPLC technique to determine monosaccharide composition of produced exopolysaccharide , I have molar ratios of 0.12 : 0.1: 0.03: 02: 0.06 of Rhamnose, Xylose, Arabinose, Manose+fructose and...

17 March 2020 2,554 3 View

How much H2SO4 is used for hydolysis of exopolysaccharide to characterize monosaccharide composition?

To characterize monosaccharide composition of exopolysaccharide by HPLC method I need to hydrolyze EPS with 0.5 M H2SO4 at 95°C for 12 h followed by neutralization with 4 M NaOH. How much sample...

05 December 2019 5,011 2 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...

30 July 2024 3,849 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

Looking for a bioinformatics expert especially one who has expertise in Nanoparticles docking ?

Silver Nanoparticles docking with alpha amylase and alpha glusidase .

11 July 2024 6,883 2 View