I was following this chip-seq protocol https://www.cellsignal.com/products/chip-kits-reagents/plus-sonication-chromatin-ip-kit/56383

what will be the consequence if on the following state "VI. Elution of Chromatin from Antibody/Protein G Magnetic Beads and Reversal of Cross-links", i perform

>step 7 " To all tubes, including the 2% input samples from Step 1, reverse cross-links by adding 6 µl 5M NaCl #7010 and 2 µl Proteinase K #10012, and incubate 2 hr at 65°C."

> before

>step 5 "Pellet Protein G Magnetic Beads by placing the tubes in a magnetic separation rack and wait 1 to 2 min for the solution to clear."

Please, I am looping forward to reading your thoughts.

thanks in advance