Hi,
I am trying to extract total RNA from chondrogenic micromass after 14days of chondrogenesis. I have used Qiagen RNAesay plus mini kit. It was extremely difficult to dissolve the micromass in RLT plus buffer. I used qiashredder as well. The RNA yield was extremely low.
I have also tried trizol extraction. Again it was difficult to get the micomass dissovled into trizol. My goal is to check for chondrogenic markers by qPCR.
If any of you have experience of RNA extraction from micromass and could share with me, that would be great.
Thanks,
Nandina.
You can crush tissue with small mortar and pestle, tissue under liquid Nitrogen all the time till get into powder. You may need to pool many micromass in one group,
Alternatetively if you want more ex vivo tissue generated, you can generate disc cartilages instead of micromass (see my paper 2016 on my RG
Thank you for your suggestion! I will try homogenization or crushing the micromass.
Regards,
Nandina.
- Badges
- Science topic
- Similar topics
- Indexes