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Questions related from Pratibha Srivastava
Is there a different methodology in mouse and human expression. I need to check the expression of a gene under the influence of two proteins. Thanking you.
01 January 2019 3,643 1 View
I prepared the recombinant protein X-FLAG tagged and two of its mutant. I have checked the mutant was correctly assembled in the pShuttle vector but cannot discover its expression even after 1...
12 December 2018 3,309 17 View
I tried twice with HA-tagging. The construct is made using pShuttle vector but failed in overexpressing the construct.
05 May 2018 6,326 5 View
Please suggest your opinion!
02 February 2018 3,279 6 View
I have tried by breaking the protein into multiple fragment and using the PCR products to clone one by one. I successfully cloned into the pShuttle vector but I couldnt get the expressed protein,...
02 February 2018 6,074 2 View