Pratibha Srivastava

5 Questions 86 Answers 0 Followers

Questions related from Pratibha Srivastava

Can anyone explain me how to design tet-on and tet-off system in mouse and human cell line?

Is there a different methodology in mouse and human expression. I need to check the expression of a gene under the influence of two proteins. Thanking you.

01 January 2019 3,625 1 View

Why do the mutants aren't expressed in the 293 cells whereas Full-length WT construct is expressed ?

I prepared the recombinant protein X-FLAG tagged and two of its mutant. I have checked the mutant was correctly assembled in the pShuttle vector but cannot discover its expression even after 1...

12 December 2018 3,296 17 View

Overexpression construct: I have 6.5kbp size protein for overexpression tool in 293 cells?

I tried twice with HA-tagging. The construct is made using pShuttle vector but failed in overexpressing the construct.

05 May 2018 6,305 5 View

What is the percentage of mutation rate in a cloning of a recombinant protein? How do you proceed for BL21, once the DH5a positive clone is achived?

Please suggest your opinion!

02 February 2018 3,263 6 View

I want to prepare an overexpression module for my protein with a N-ter HA tag. Can you suggest me the possible strategies?

I have tried by breaking the protein into multiple fragment and using the PCR products to clone one by one. I successfully cloned into the pShuttle vector but I couldnt get the expressed protein,...

02 February 2018 6,061 2 View