Why most of the studies use cisplatin i.p injection at dose 3 mg/kg?

I will start a cisplatin treatment soon for NU/NU Nude Mice (Crl:NU-Foxn1nu) and I found multiple publications using 3 mg/kg i.p injection with nude mice. There were different injection frequency...

11 December 2018 5,028 2 View

In Springer publications, my article has two status for two weeks now without any further contacts from them?

My article in Medical Oncology is in two places "submission with decision" which is accept and in "submission with production complete". We didn't get any mails from them though. Any one had the...

06 July 2018 4,886 0 View

Why suspension culture and serum free conditions are used to isolate cancer stem cells?

Tumor sphere formation have been used lately to isolate cancer stem cells and proliferate them. why the non-stem cells don't survive these conditions?

05 June 2018 979 5 View

Why serum free conditions are used to isolate cancer stem cells?

I am asking what is the specific criteria that makes stem cells survive the serum free medium (supplemented with B27, N2, FGF and EGF) and the non-stem cells die?

05 June 2018 7,182 4 View

What is the difference between stemness and self-renewal?

In cancer stem cells it is always mentioned that they have stemness properties and self-renewal ability. Are those the same thing but different names or there is a difference?

04 May 2018 9,544 4 View

Anyone has a protocol for chondrogenic differentiation of MSCs in monolayer ? and a method of quantification of the results?

I am trying to avoid the pellet protocol

04 May 2017 7,481 0 View

In sphere forming test, should i change the medium during the test period? and if yes how to do that in low attachment plates?

09 October 2016 10,022 0 View

I need to do the Transwell migration and invasion assay on squamous cell carcinoma cell line. anyone know the protocol and where can i get the plates?

working on SCC12 cell line where i can get the Matrigel?

07 August 2016 1,023 2 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

No title

01 March 2021 3,905 5 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View