Osama Elkashty

9 Questions 12 Answers 0 Followers

Questions related from Osama Elkashty

Why most of the studies use cisplatin i.p injection at dose 3 mg/kg?

I will start a cisplatin treatment soon for NU/NU Nude Mice (Crl:NU-Foxn1nu) and I found multiple publications using 3 mg/kg i.p injection with nude mice. There were different injection frequency...

12 December 2018 5,129 2 View

In Springer publications, my article has two status for two weeks now without any further contacts from them?

My article in Medical Oncology is in two places "submission with decision" which is accept and in "submission with production complete". We didn't get any mails from them though. Any one had the...

07 July 2018 4,975 0 View

Why serum free conditions are used to isolate cancer stem cells?

I am asking what is the specific criteria that makes stem cells survive the serum free medium (supplemented with B27, N2, FGF and EGF) and the non-stem cells die?

06 June 2018 7,356 4 View

Why suspension culture and serum free conditions are used to isolate cancer stem cells?

Tumor sphere formation have been used lately to isolate cancer stem cells and proliferate them. why the non-stem cells don't survive these conditions?

06 June 2018 1,103 5 View

What is the difference between stemness and self-renewal?

In cancer stem cells it is always mentioned that they have stemness properties and self-renewal ability. Are those the same thing but different names or there is a difference?

05 May 2018 9,680 4 View

Anyone has a protocol for chondrogenic differentiation of MSCs in monolayer ? and a method of quantification of the results?

I am trying to avoid the pellet protocol

05 May 2017 7,576 0 View

In sphere forming test, should i change the medium during the test period? and if yes how to do that in low attachment plates?

I try to do the sphere forming assay with oral squamous cell carcinoma cells to test the effect of a drug against cancer stem cells.

10 October 2016 10,114 0 View

I need to do the Transwell migration and invasion assay on squamous cell carcinoma cell line. anyone know the protocol and where can i get the plates?

working on SCC12 cell line where i can get the Matrigel?

08 August 2016 1,149 2 View

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)?

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)? need to get 5 fluorouracil as a chemotherapy for SCC

07 July 2016 5,852 6 View