Why most of the studies use cisplatin i.p injection at dose 3 mg/kg?

I will start a cisplatin treatment soon for NU/NU Nude Mice (Crl:NU-Foxn1nu) and I found multiple publications using 3 mg/kg i.p injection with nude mice. There were different injection frequency...

11 December 2018 5,028 2 View

Why suspension culture and serum free conditions are used to isolate cancer stem cells?

Tumor sphere formation have been used lately to isolate cancer stem cells and proliferate them. why the non-stem cells don't survive these conditions?

05 June 2018 979 5 View

Why serum free conditions are used to isolate cancer stem cells?

I am asking what is the specific criteria that makes stem cells survive the serum free medium (supplemented with B27, N2, FGF and EGF) and the non-stem cells die?

05 June 2018 7,182 4 View

What is the difference between stemness and self-renewal?

In cancer stem cells it is always mentioned that they have stemness properties and self-renewal ability. Are those the same thing but different names or there is a difference?

04 May 2018 9,544 4 View

Anyone has a protocol for chondrogenic differentiation of MSCs in monolayer ? and a method of quantification of the results?

I am trying to avoid the pellet protocol

04 May 2017 7,481 0 View

In sphere forming test, should i change the medium during the test period? and if yes how to do that in low attachment plates?

09 October 2016 10,022 0 View

I need to do the Transwell migration and invasion assay on squamous cell carcinoma cell line. anyone know the protocol and where can i get the plates?

working on SCC12 cell line where i can get the Matrigel?

07 August 2016 1,023 2 View

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)?

What is the best solvent i can use to prepare Floxuridine working solution (will be used on cell lines and animals)? need to get 5 fluorouracil as a chemotherapy for SCC

06 July 2016 5,772 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View