Do you have experience with Qiagen LNA probes for 3'UTR detection with RNA FISH?

06 March 2023 0 7K Report

Hello,

I am new in RNA FISH and I want to use Qiagen LNA probes that I designed through GeneGlobe tool of the company. The probes are 20-22 in size and the site gives no chance to increase. Is this length proper for CDS or 3'UTR site detection of the transcripts? Can I be sure about specifity? I checked the literature and saw that these probes are usually used for miRNAs. I am a bit confused. Do you have any other suggestions or experience?

I appreciate your help!

Melis

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